Posttranscriptional Regulation by the Upstream Open Reading Frame of the Phosphoethanolamine N-Methyltransferase Gene

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概要

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• Phosphoethanolamine N-methyltransferase (PEAMT) is involved in choline biosynthesis in plants. The 5′ untranslated region (UTR) of several PEAMT genes was found to contain an upstream open reading frame (uORF). We generated transgenic Arabidopsis calli that expressed a chimeric gene constructed by fusing the 5′ UTR of the Arabidopsis PEAMT gene (AtNMT1) upstream of the β-glucuronidase gene. The AtNMT1 uORF was found to be involved in declining levels of the chimeric gene mRNA and repression of downstream β-glucuronidase gene translation in the calli when the cells were treated with choline. Further, we discuss the role of the uORF.

• 社団法人 日本農芸化学会の論文
• 2007-09-23

著者

• Okada Tomoyuki

  Laboratory Of Tropical Plant Science Faculty Of Agriculture Kobe University

• Azuma Tetsushi

  Graduate School Of Science And Technology Kobe University

• Yasuda Takeshi

  Laboratory Of Tropical Plant Science Faculty Of Agriculture Kobe University

• TABUCHI Tomoki

  Laboratory of Tropical Plant Science, Faculty of Agriculture, Kobe University

• NANMORI Takashi

  Graduate School of Science and Technology, Kobe University

• Tabuchi Toshihito

  Laboratory Of Tropical Plant Science Faculty Of Agriculture Kobe University

• Nanmori Takashi

  Graduate School Of Science And Technology Kobe University

• Nanmori Takashi

  Graduate School Of Sci. & Tech. Div. Of Mol. Sci. Kobe Univ.

• Azuma Tetsushi

  Graduate School Of Agricultural Science Kobe University

• Yasuda Takeshi

  Graduate School Of Sci. & Tech. Kobe Univ.

• Nanmori Takashi

  Graduate School of Agricultural Science, Kobe University

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