C57BL/6J遺伝的背景の高効率FLPeディリーターマウス
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概要
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The increasing popularity of conditional knockout (KO) technology has resulted in the demand for efficient FLP deleter mice. In addition, FLP deleters are needed in genetic backgrounds that are suited to behavioral studies. We generated CAG-FLPe transgenic (Tg) mice with the C57BL/6J genetic background, which is one of the most commonly-used strains in behavioral studies. We assessed the recombination efficiency of the CAG-FLPe-Tg lines by crossing them with a mouse line carrying a FRT-PGK-neo-FRT cassette. Four of five independent CAG-FLPe lines induced recombination in most (91%-100%) of their progenies, although a small fraction (0%-30%, depending on the line) showed mosaic recombination patterns. These animals are highly potent as deleters of FRT cassettes and are useful for behavioral studies involving conditional KO mice.
- 2006-04-01
著者
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KANKI Hiroaki
Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Che
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SUZUKI Hitomi
Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Che
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ITOHARA Shigeyoshi
Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Che
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Suzuki Hitomi
Laboratory For Behavioral Genetics Brain Science Institute (bsi) The Institute Of Physical And Chemi
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Kanki Hiroaki
Laboratory For Behavioral Genetics Brain Science Institute (bsi) The Institute Of Physical And Chemi
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Itohara Shigeyoshi
Laboratory For Behavioral Genetics Advanced Technology Development Center Riken Brain Science Instit
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Itohara Shigeyoshi
Laboratory For Behavioral Genetics Brain Science Institute (bsi) The Institute Of Physical And Chemi
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神吉 浩明
Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Chemical Research (RIKEN)
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鈴木 瞳
Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Chemical Research (RIKEN)
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糸原 重美
Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Chemical Research (RIKEN)
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- C57BL/6J遺伝的背景の高効率FLPeディリーターマウス
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