Production in Escherichia coli and Application of a Recombinant Carbonic Anhydrase of the Cyanobacterium Anabaena sp. Strain PCC7120
スポンサーリンク
概要
- 論文の詳細を見る
The production and low-temperature application of cyanobacterial carbonic anhydrase that catalyzes the reversible hydration between CO<SUB>2</SUB> and HCO<SUB>3</SUB><SUP>–</SUP> has been studied. The genes encoding carbonic anhydrase was isolated from the cyanobacterium <I>Anabaena</I> sp. strain PCC7120 that performs a photosynthesis in a wide range of temperature and even at around 288 K, and functionally expressed it in <I>Escherichia coli</I> Origami B(DE3) using an expression vector pET15b. The specific activity reached 528 WA (Wilbur–Anderson units per mg of protein) at 298 K, which was 2.5 times higher than that at 310 K. Addition of this recombinant carbonic anhydrase to the aerated culture of the cyanobacterium <I>Synechococcus</I> sp. strain PCC7942/1 at 293 K increased the carbon dioxide fixation rate 1.9 fold to that without its addition.
- 2006-03-01
著者
-
Ohtaguchi Kazuhisa
Dep. Of Chemical Engineering Tokyo Inst. Of Technol.
-
Ohtaguchi Kazuhisa
Department Fo Chemical Engineering Tokyo Institute Of Technology
-
OGATA Naohisa
Department of Chemical Engineering, Tokyo Institute of Technology
-
Ogata Naohisa
Department Of Chemical Engineering Tokyo Institute Of Technology
関連論文
- The Kinetics of Growth and the CO_2 Concentrating Mechanism of the Filamentous Cyanobacterium Anabaena cylindrica in a Bubble Column
- Kinetics of the Growth of White-Rot Fungus Coriolus hirsutus in Soil for Bioremediation
- GAS HOLDUP AND VOLUMETRIC LIQUID-PHASE MASS TRANSFER COEFFICIENT IN A GEL-PARTICLE SUSPENDED BUBBLE COLUMN WITH DRAUGHT TUBE
- Biosynthesis of Polysaccharide Alginate by Azotobacter vinelandii in a Bubble Column
- CONTINUOUS PRODUCTION OF ACETIC ACID FROM CO_2 IN REPEATED-BATCH CULTURES USING FLOCCULATED CELLS OF ACETOBACTERIUM WOODII
- CORRELATIONS BETWEEN OCTANOL/WATER PARTITION COEFFICIENTS AND INTRINSIC MOLECULAR PROPERTIES OF SIMPLE ORGANIC COMPOUNDS
- COMPUTER-AIDED PREDICTION OF SOLUBILITIES OF ORGANIC COMPOUNDS IN WATER
- CORRELATION AND PREDICTION OF AUTOIGNITION TEMPERATURES OF HYDROCARBONS USING MOLECULAR PROPERTIES
- QUANTITATIVE ANALYSIS OF FLOCCULATION OF ACETOBACTERIUM WOODII BY CHEMICAL FLOCCULANTS
- CORRELATION BETWEEN SOLUBILITIES IN WATER AND MOLECULAR DESCRIPTORS OF HYDROCARBONS
- KINETICS OF PRODUCTION OF ACETIC ACID FROM CARBON DIOXIDE BY ACETOBACTERIUM WOODII IN BUBBLE-COLUMN BIOREACTOR
- EFFCTS OF ALCOHOL AND PARTICLE CONCENTRATIONS ON LONGITUDINAL LIQUID DISPERSION COEFFICIENT IN GEL-PARTICLE-SUSPENDED BUBBLE COLUMN
- EFFECTS OF ALCOHOLS ON GAS HOLDUP AND VOLUMETRIC LIQUID-PHASE MASS TRANSFER COEFFICIENT IN GEL-PARTICLE-SUSPENDED BUBBLE COLUMN
- CHLOROPLAST STRUCTURE AND SUB-CELLULAR DISTRIBUTION OF α-LINOLENIC ACID IN THE HOT-SPRING ALGA CYANIDIUM CALDARIUM
- CARBON DIOXIDE FIXATION AND α-LINOLENIC ACID PRODUCTION BY THE HOT-SPRING ALGA CYANIDIUM CALDARIUM
- Metabolism of L-Amino Acids in a Marine Bacterium Isolated from Mackerel Intestines in Relation to Eicosapentaenoic Acid Biosynthesis
- Size-Effects in the Separation of Bovine Serum Albumin through an Aqueous Polymer Two-Phase Systems on the Micron-Size Devices
- EFFECTS OF AERATION RATE AND PRECULTURE AGE ON BATCH GROWTH OF TRIPTERYGIUM WILFORDII IN BUBBLE-COLUMN BIOREACTORS
- Production of Hydrogen by Thermophilic Cyanobacterium Synechococcus sp. strain H-1
- Shortening the Light Path Length of Photobioreactors to Elevate the Cell Mass Concentration of Cyanobacterium Synechococcus sp. Strain PCC6301
- Kinetics of Biofilm Formation of a Cyanobacterium on Bioreactor Surfaces
- In situ FT-IR Study on the Transient States of Adsorbed Species during CO Oxidation by O_2 and NO over Pt/γ-Al_2O_3 Catalyst
- Production in Escherichia coli and Application of a Recombinant Carbonic Anhydrase of the Cyanobacterium Anabaena sp. Strain PCC7120
- Chemometrics approach to the analysis of a synergism of temperature, light intensity and carbon dioxide concentration on the growth of Anacystis nidulans.
- Effect of Oxygen Mass Transfer between the Gas and Liquid Phases on the Production of Monascus Red Pigments by Monascus ruber
- Effects of Starch and Protein on Glucosamine Content in the Biomass of Monascus ruber
- Determination of functional groups in glucose isomerase.
- Relation between Hyphal Growth and Red Pigment Production of the Fungus Monascus ruber NBRC 32318 on Rice Starch Agar
- Call Growth Inhibition in Mouse Leukemia L5178Y Cells in Vitro by Crude Extracts from Freshwater Blue-green Algae.