Identification of Calcitic and Aragonitic Otoconia by Selective Staining Methods

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概要

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• This report concerns whether the otoconia of the utricle and saccule removed from the inner ear of amphibia, especially of the frog <I>Rana nigromaculata</I>, are composed of calcite or aragonite. These compounds were identified by selective staining with alizarine red S, Harris hematoxylin and Feigls solution. The result showed that both the saccular and the utricular otoconia were stained red and purple with alizarine red S and Harris hematoxylin, respectively, while only the saccular otoconia were stained by black precipitates with Feigls solution which stains aragonite. Furthermore, mineral aragonite, which is an inorganic substance, was stained with Feigls solution for the purpose of comparison with the saccular otoconia, which contain organic substances. Thus, the saccular otoconia and the mineral aragonite had the same staining appearance. These observations provide evidence that the saccular otoconia consist of aragonite, the utricular of calcite. The present, selective staining methods may be useful for studies, such as those focused on phylogenetic development, of calcitic and aragonitic otoconia in otolithic organs.

• 1996-04-01

著者

• Kido Toshishige

  Department Of Otolaryngology Yamaguchi University School Of Medicine

関連論文

• Identification of Calcitic and Aragonitic Otoconia by Selective Staining Methods
• External Auditory Canal Cholesteatoma : Surgical Treatment Using Free Grafts of Auricular Conchal Cartilage and Temporal Fascia

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