Effect of Retinoic Acid on Murine Preosteoblastic MC3T3-E1 Cells
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概要
- 論文の詳細を見る
Retinoic acid (RA) plays an important role in bone metabolism in vivo through osteoclast activation and bone resorption. Retinoid X-activated receptor β(RXRβ) has been implicated in the genetic spinal defect of ossification of the posterior longitudinal ligament (OPLL). In this study, we examined the effects of 9-cis RA and all-trans RA (ATRA) on the proliferation, differentiation, and RXRβ expression of the murine preosteoblastic cell line MC3T3-E1. Both 9-cis RA and ATRA dose-dependently inhibited the increase in total soluble protein content at concentrations of 10 and 100nM after 4 and 8d co-culture with MC3T3-E1 cells. The inhibitory effect of 9-cis RA was slightly stronger than that of ATRA. Histone H4 mRNA expression was dose-dependently suppressed by both RAs on day 1. Alkaline phosphatase activity was increased by both RAs at 10 and 100nM concentrations on day 4, with 9-cis RA-induced activity slightly stronger than that of ATRA. Osteopontin mRNA expression was increased by both RAs on day 1, but was suppressed on day 4. Bone Gla protein mRNA expression was inhibited by 10 and 100nM 9-cis RA and by 100nM ATRA on day 14. RXRβ mRNA expression was increased by 9-cis RA, an RXRβ ligand, in a dose-dependent manner. Our results suggested that while both RAs suppressed proliferation and stimulated the maturation of preosteoblastic MC3T3-E1 cells, 9-cis RA was slightly more potent than ATRA. It also appeared that RAs may contribute to the development of heterotopic ossification, including OPLL.
- 日本ビタミン学会の論文
著者
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Sato Kozo
Department of Diagnostic Radiology, Keio University School of Medicine
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KOBAYASHI Akira
Department of Biochemistry, Tohoku University School of Medicine
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Sato Kozo
Department Of Diagnostic Radiology Keio University School Of Medicine
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Tazawa Hiroshi
Department Of Orthopedic Surgery Akita University School Of Medicine
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Sato Kozo
Department Of Orthopedic Surgery Akita University School Of Medicine
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Tazawa Hiroshi
Department Of Orthopaedic Surgery Akita University School Of Medicine
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NAGASAWA Hiroyuki
Department of Orthopedic Surgery, Akita University School of Medicine
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TAKAHASHI Shu
Department of Orthopedic Surgery, Akita University School of Medicine
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TASHIMA Yohtalou
Department of Biochemistry, Akita University School of Medicine
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TASHIMA Yohtalou
Second Department of Biochemistry Akita University School of Medicine
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Tashima Yohtalou
Department Of Biochemistry Akita University School Of Medicine
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Takahashi Shu
Department Of Orthopedic Surgery Akita University School Of Medicine
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Komatsuda A
Second Department Of Biochemistry Akita University School Of Medicine
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Nagasawa Hiroyuki
Department Of Industrial Engineering Graduate School Of Engineering Osaka Prefecture University
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Sato Kozo
Department Of Anesthesiology Institute Of Clinical Medicine University Of Tsukuba
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Nagasawa Hiroyuki
Department Of Industrial Engineering College Of Engineering Osaka Prefecture University
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Takahashi Shu
Department Of Molecular Medicine Sapporo Medical University
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Kobayashi Akira
Department 9 Electron Tube Division #2 Hamamatsu Photonics K.k.
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