Avena Rhealba^[○!R] Inhibits A23187-Stimulated Arachidonic Acid Mobilization, Eicosanoid Release, and cPLA_2 Expression in Human Keratinocytes : Potential in Cutaneous Inflammatory Disorders(Pharmacology)

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The aim of the present study was to examine the effects of Avena Rhealba^<[○!R]> (AR) oatmeal extract on the metabolism of arachidonic acid (AA) and eicosanoids as well as on the expression of cytosolic phospholipase A_2 (cPLA_2) in the human keratinocyte cell line HaCaT. For this purpose, we examined the effects of AR on basal and A23187-triggered release of [^3H]-AA from phospholipids and on the production of [^3H]-labeled metabolites of the cyclooxygenase (CO) and 5-lipoxygenase (LO) pathways. AR was found to inhibit A23187-triggered [^3H]-AA mobilization from phospholipids (p<0.05) and production of [^3H]-labeled metabolites of CO (p<0.05) and LO (p<0.05) pathways. These results suggest AR decreases PLA_2-dependent mobilization of AA from phospholipids. A closer examination of the effects of AR on prostaglandin 6KF1α (6KPGF1α), the stable metabolite of prostacyclin, revealed dose-dependent inhibition of this AA metabolite. AR also decreased A23187- and tumor necrosis factor α-induced cPLA_2 overexpression, as shown by cPLA_2 immunodetection and mRNA expression. These results demonstrate the high potential of AR in the treatment of inflammatory diseases of the skin.
公益社団法人日本薬学会の論文
2005-04-01