抜歯創傷治癒での骨新生における TGF-βの役割

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TGF-β has been shown to have the potential to stimulate bone formation. The purpose of this study is to examine the role of TGF-β in bone formation in the extraction cavity after tooth extraction in mice. First, the enzyme-positive area was examined histochemically for alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRAP) was examined in the sections of the decalcified tooth and periodontal tissue of mice from the first day to the 14th day of tooth extraction. The TRAP-positive areas remained at a low level during the first 5 days, rapidly increased on 7th day, and remained at a high level thereafter. The ALP-positive areas increased similarly, but with a log of 3 days. Anti-TGF-β neutralizing antibodies were injected in the submucosal tissue near the extraction cavity on an appropriate day between the first and the 14th day after tooth extraction. The effect of anti-TGF-β antibodies on trabecular bone area, ALP-positive area, or TRAP-positive area in the extraction cavity was examined using frozen sections from the decalcified periodontal tissues of the mice 12 days after tooth extraction. In the case of ALP staining, the enzyme-positive area significantly increased in sections from the mice injected with the antibodies 3 days or 7 days after tooth extraction. The ALP-positive area appeared to involve a decreasing tendency in the case of injection on the 5th day after tooth extraction. In the case of TRAP staining, the enzyme-positive area appeared to involve be a decreasing tendency in the sections from the mice injected with the antibodies 10days after tooth extraction. The trabecular bone area was significantly decreased in the sections from the mice injected with the antibodies 3 days after tooth extraction compared to those injected with saline on the same day. There was no significant difference between the other sections described above in the antibody injection group and those in the saline injection group. Thus, the above results suggest that the role of TGF-β in bone formation depends on the number of day after tooth extraction.
九州歯科学会の論文
2000-02-25

抜歯創傷治癒での骨新生における TGF-βの役割

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