思春期ラットの虚弱骨における食餌療法に関する酵素組織化学的および組織形態計測学的研究 : 骨基質形成

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The influence of diet therapy on bone matrix formation was examined in the tibial metaphysis by endochondral ossification. Eight-week-old male Wistar rats corresponding to humans during adolescence were fed a calcium-deficient diet (0.01mg/100mg) or a low-calcium diet (144mg/100g) for 6 weeks, or the same diet for 3 weeks to induce a fragile state, followed by a standard diet for 3 weeks as a diet regimen. The following results were obtained. 1. Histopathologic findings showed a wider chondrocyte layer, decreased calcified matrix, and reduced erosion of the calcified matrix in the thickened zone chondrocytes and columnar gaps, particularly in the group fed a calcium-deficient diet compared with those in the control group. In the group fed low-calcium and standard diets, the width of the chondrocyte layer was reduced, and the calcified matrix and erosion of the calcified matrix in the columnar gaps were increased, which were findings similar to those in the control group, indicating facilitation of bone matrix formation by endochondral ossification. 2. In enzymohistochemical findings, ALP-positive cells indicating osteoblasts on the surface of the osseous beam continuing from the new osseous beam toward the lower part in the group fed low-calcium and standard diets were similar to those in the control group. TRAP-positive cells indicating osteoclast-like cells in the group fed low-calcium and standard diets were similar to those in the control group. 3. Histomorphometric findings demonstrated that the number of osteoclasts per unit area of the secondary cancellous bone was significantly increased in the order of the group fed a low-calcium diet<the group fed calcium-deficient and standard diets<the group fed a calcium-deficient diet, compared with that in the control group. However, there was no significant difference between the group fed low-calcium and standard diets and the control group. This study performed histopathologic examination, enzymohistochemical examination, and measured the number of osteoclast-like cells concerning bone resorption of bone metastasis as histomorphometry for quantification, to concretely examine the effects of diet therapy by administering calcium for bone in a fragile state on bone matrix formation. Consequently, bone that had been in a fragile state during adolescence, particularly in the group fed low-calcium and standard diets, showed almost the same findings as those in the control group after balanced diet therapy with calcium, demonstrating recovery of bone matrix formation by endochondral ossification.
九州歯科学会の論文
2000-02-25

思春期ラットの虚弱骨における食餌療法に関する酵素組織化学的および組織形態計測学的研究 : 骨基質形成

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