培養癌細胞による抗癌剤の効果を高めるための実験的研究 : KB 細胞に対するシスプラチンとカフェインおよびカルシトニンとの併用効果
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概要
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In the chemotherapy for head and neck cancers, cisplatin (CDDP) has exellent antitumor effects and is necessary for combination therapy. At the same time, this anticancer drug has various side effects. In an attempt to minimize the side effects of CDDP and to enhance antitumor effects, CDDP is combined with non-anticancer drugs and administered. For the cultivated cancer cells, KB cells were used and cultivated for a fixed period. Then, CDDP is combined with Caffeine (Caff) or synthetic eel calcitonin (ECT) and administered to the cells. Their effects on the KB cells were investigated in terms of growth conditions and morphology. The results were as follows : 1. No significant antitumor effect in terms of growth condition was found in the CDDP alone (1μg/ml) group (group B1), as compared with the control group. However, between the CDDP alone (2μg/ml) group (group B2) and the control group, significant antitumor effect in terms of growth condition and cytomorphologic changes were found. 2. Between the Caff alone (100μg/ml, or 200μg/ml) groups (group C1, C2) and the control group, no significant genesistasis nor marked cytomorphologic changes were shown. 3. Between the ECT alone (1/50U/ml, or 1/100U/ml) groups (group D1, D2) and the control group, no significant difeerences in genesistasis nor in cytomorphologic changes were shown. 4. Between the CDDP-Caff combined groups (group E1, E2) and control goup, significant differences in genesistasis (p<0.01) and marked cytomorphologic changes were shown. However, between group E1 and group E2, each of 2- and 3-day groups did not show significant genesistasis. 5. The CDDP-ECT combined groups (group F1, F2) showed significant genesistasis (p<0.01) and marked cytomorphologic chainges as compared with the control group. However, between group F1 and group F2, no significant genesistasis was shown. 6. On the basis of the foregoing results, the combined application of CDDP and Caff or ECT enhanced antitumor effect several times. The present experiment results gave considerable suggestions for the clinical aspect, although many further experimental studies need to be made.
- 1999-02-25