Effect of Bcl-2 Overexpression on Cell Cycle and Antibody Productivity in Chemostat Cultures of Myeloma NS0 Cells(CELL AND TISSUE ENGINEERING)

概要

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Chemostat cultures of NS0 cell lines were carried out at dilution rates ranging from 0.8d^<-1> to 0.2d^<-1>. Compared with the control, the viable cell density of the Bcl-2 cell line was approximately 10% higher at 0.8d^<-1> and increased to 55% when the dilution rate was reduced to 0.2d^<-1>. As the dilution rate was reduced, the viability of the two cultures diverged reaching a difference of 43% at 0.2d^<-1>. The specific growth rate of the control cells was the same as the dilution rate down to a value of 0.6d^<-1>. By contrast, the specific growth rate of Bcl-2 cells was parallel to the dilution rate down to a value as low as 0.3d^<-1>. For both NS0 cell lines, the G_1 cell population decreased, while the S and G_2/M cell populations increased as the dilution rate was reduced. The antibody titer of the control cells increased from 7 to 21μg・ml^<-1> as the dilution rate was reduced from 0.8 to 0.2d^<-1>. With an initial increase from 2 to 15μg・ml^<-1> as the dilution rate was reduced from 0.8 to 0.4d^<-1>, the antibody titer of the Bcl-2 cells remained constant as the dilution rate was further reduced to 0.2d^<-1>. A good correlation between specific antibody production rate and the percentage of G_2/M cells was observed.
公益社団法人日本生物工学会の論文
2005-09-25