Evaluation of Solution Phase Isoelectrofocusing as Part of Proteomics Strategies
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概要
- 論文の詳細を見る
Solution isoelectrofocusing has been evaluated for prefractionation of nuclear proteins from human cancer MCF-7 cells. Two separation devices were exercised using commercial polyacrylamide membranes, which carry embedded immobiline mixtures to establish pH boundaries between chambers. Protein recovery was quantified and 2-D gel electrophoresis was used to evaluate resolution. Solution isoelectrofocusing provides satisfactory separations and decreases the complexity of the protein mixture, however rates of recovery need to be improved.
- 日本質量分析学会の論文
- 2005-02-01
著者
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Fenselau Catherine
Department Of Chemistry & Biochemistry And Marlene & Stewart Greenebaum Cancer Center Univer
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Fenselau Catherine
Department Of Chemistry & Biochemistry University Of Maryland
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AN Yanming
Department of Chemistry & Biochemistry, University of Maryland
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FU Zongming
Department of Chemistry & Biochemistry, University of Maryland
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An Yanming
Department Of Chemistry & Biochemistry University Of Maryland
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Fu Zongming
Department Of Chemistry & Biochemistry University Of Maryland
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- Evaluation of Solution Phase Isoelectrofocusing as Part of Proteomics Strategies