Programmed Cell Death Detected in Interspecific Hybrids of Nicotiana repanda × N. tomentosiformis Expressing Hybrid Lethality
スポンサーリンク
概要
- 論文の詳細を見る
Interspecific hybrids of Nicotiana repanda × N. tabacum die shortly after germination. Seedlings that are hybrids between N. repanda and N. tomentosiformis, which is a progenitor of N. tabacum, express hybrid lethality when they are cultured at 28 ℃ for 90 days after germination, but seedlings that are hybrids between N. repanda and N. sylvestris, another progenitor of N. tabacum, grow normally. We detected several features of programmed cell death (PCD) in hybrid seedlings of N. repanda × N. tomentosiformis expressing hybrid lethality. Nuclear fragmentation was detected in nuclei isolated from dying shoots and roots of hybrid seedlings expressing lethality. In addition, electrophoresis of total DNA extracted from the shoots and roots of hybrid seedlings showed a distinctive DNA ladder pattern, suggesting cleavage of nuclear DNA into oligonucleosomal fragments. These results indicate that hybrid seedlings of N. repanda × N. tomentosiformis undergo PCD in the process of hybrid lethality. However, in hybrid seedlings of N. repanda × N. sylvestris, no features of PCD were detected. These results suggest that PCD might also be expressed in hybrid seedlings of N. repanda × N. tabacum because N. tomentosiformis is a progenitor of N. tabacum.
- 日本育種学会の論文
著者
-
Kobori Satoshi
School Of Agriculture Ibaraki University
-
Marubashi W
School Of Agriculture Meiji University
-
Marubashi Wataru
School Of Agriculture Ibaraki University
関連論文
- Developmental Observation and High Temperature Rescue from Hybrid Weakness in a Cross between Japanese Rice Cultivars and Peruvian Rice Cultivar 'Jamaica'
- High levels of the cytokinin BAP suppress programmed cell death in hybrid tobacco cells (Nicotiana suaveolens×N. tabacum) expressing hybrid lethality
- Apoptotic Cell Death Observed during the Expression of Hybrid Lethality in Interspecific Hybrids between Nicotiana tabacum and N. suaveolens
- Anthocyanins of Gladiolus Cultivars and their Contribution to Flower Colors
- Characterization and Overcoming of Temperature-Dependent Lethality Exhibited by Hybrid Embryos from the Cross Nicotiana suaveolens × N.sylvestris
- Efficiency of Inter-Simple Sequence Repeat PCR for Detecting Somaclonal Variation among Leaf-Culture-Regenerated Plants of Horseradish
- P-07. Comparison of ribosomal DNA sites on somatic chromosomes of two Lobelia species(Abstracts of the oral and poster presentations, Abstracts of the 54th Annual Meeting of the Society of Chromosome Research)
- Heat treatment temporarily suppresses expression of programmed cell death in hybrid tobacco cells (Nicotiana suaveolens×N. tabacum) expressing hybrid lethality
- Flavonols concerned in flower coloration of Gladiolus cultivars
- Novel Evaluation Method of Flower Senescence in Freesia (Freesia hybrida) Based on Apoptosis as an Indicator
- Possible Involvement of Auxin-Induced Ethylene in an Apoptotic Cell Death during Temperature-Sensitive Lethality Expressed by Hybrid between Nicotiana glutinosa and N. repanda
- Facile Induction of Apoptosis into Plant Cells Associated with Temperature-Sensitive Lethality Shown on Interspecific Hybrid from the Cross Nicotiana suaveolens x N. tabacum
- Detection of Four Lethality Types in Interspecific Crosses among Nicotiana Species through the Use of Three Rescue Methods for Lethality
- Time Course Analysis of Apoptotic Cell Death during Expression of Hybrid Lethality in Hybrid Tobacco Cells (Nicotiana suaveolens × N. tabacum)
- Even juvenile leaves of tobacco exhibit programmed cell death
- Programmed Cell Death Detected in Interspecific Hybrids of Nicotiana repanda × N. tomentosiformis Expressing Hybrid Lethality
- Development of Q-chromosome-specific DNA Markers in Tobacco and Their Use for Identification of a Tobacco Monosomic Line
- Temperature-Dependent Programmed Cell Death Detected in Hybrids between Nicotiana langsdorffii and N. tabacum Expressing Lethality
- Temperature-dependent Apoptosis Detected in Hybrids between Nicotiana debneyi and N. tabacum Expressing Lethality
- Q Chromosome Controls the Lethality of Interspecific Hybrids between Nicotiana tabacum and N. suaveolens
- Bijections between topologies