Catechol 2, 3-Dioxygenase from Pseudomonas sp. Strain ND6 : Gene Sequence and Enzyme Characterization

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概要

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• The catechol 2,3-dioxygenase (C23O) gene in naphthalene catabolic plasmid pND6-1 of Pseudomonas sp. ND6 was cloned and sequenced. The C23O gene was consisted of 924 nucleotides and encoded a polypeptide of molecular weight 36 kDa containing 307 amino acid residues. The C23O of Pseudomonas sp. ND6 exhibited 93% and 89% identities in amino acid sequence with C23Os encoded by naphthalene catabolic plasmid NAH7 from Pseudomonas putida G7 and the chromosome of Pseudomonas stutzeri AN10 respectively. The Pseudomonas sp. ND6 C23O gene was overexpressed in Escherichia coli DH 5α using the lac promoter of pUC18, and its gene product was purified by DEAE-Sephacel and Phenyl-Sepharose CL-4B chromatography. The enzymology experiments indicated that the specific activity and thermostability of C23O from Pseudomonas sp. ND6 were better than those of C23O from Pseudomonas putida G7.

• 社団法人 日本農芸化学会の論文
• 2004-08-23

著者

• Jiang Yun

  Department Of Microbiology Nankai University

• Jiang Yun

  Department Of Immunology The Wenner-gren Institute Arrhenius Laboratories For Natural Sciences Stock

• YANG Xianglong

  Department of Laboratory Science, Tianjin Medical University

• LIU Bin

  Genomics Institute, Chinese Academy of Sciences

• ZHAO Huabing

  Department of Microbiology, Nankai University

• CHENG Qiuxiang

  Department of Microbiology, Nankai University

• CAI Baoli

  Department of Microbiology, Nankai University

• Cai Baoli

  Department Of Microbiology Nankai University

• Zhao Huabing

  Department Of Microbiology Nankai University

• Yang Xianglong

  Department Of Laboratory Science Tianjin Medical University

• Cheng Qiuxiang

  Department Of Microbiology Nankai University

• Liu Bin

  Genomics Institute Chinese Academy Of Sciences

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• Catechol 2, 3-Dioxygenase from Pseudomonas sp. Strain ND6 : Gene Sequence and Enzyme Characterization

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