The Artificial Evolution of an Enzyme by Random Mutagenesis : The Development of Formaldehyde Dehydrogenase

スポンサーリンク

概要

• 論文の詳細を見る

• A unique variant of glutathione independent formaldehyde dehydrogenase of Pseudomonas putida was obtained by random mutagenesis using the PCR-reaction. This YM042 mutant, S318G, was a cold-adapted formaldehyde dehyrogenase. The activity at 29 °C of the variant was 1.7-fold higher than that of the wild type. The Km values of the mutant at 37 °C were 0.40 mM for NAD+ and 2.5 mM for formaldehyde, while those of the wild-type were 0.18 mM for NAD+ and 2.1 mM for formaldehyde. The catalytic efficiency for formaldehyde was about 1.5-fold greater in the mutant than in the wild-type enzyme. The optimum pHs and temperatures of the mutant and the wild-type enzyme were 7.5, and 8.0 and 37 °C, and 47 °C, respectively. The thermal stability of the mutant was lower than that of the wild type.

• 社団法人 日本農芸化学会の論文
• 2004-08-23

著者

• OHKUBO Katsutoshi

  Institute of Advanced Energy, Kyoto University

• Hada Megumi

  Institute Of Advanced Energy Kyoto University

• FUJII Yasuyo

  Institute of Advanced Energy, Kyoto University

• YAMASAKI Yoshiaki

  Institute of Advanced Energy, Kyoto University

• MATSUMOTO Masahiro

  Institute of Advanced Energy, Kyoto University

• NISHIDA Hiroyuki

  Institute of Advanced Energy, Kyoto University

• Fujii Yasuyo

  Institute Of Advanced Energy Kyoto University

• Yamasaki Yoshiaki

  Institute Of Advanced Energy Kyoto University

• Nishida Hiroyuki

  Institute Of Advanced Energy Kyoto University

• Ohkubo Katsutoshi

  Institute For Fundamental Organic Chemistry Kyushu University

関連論文

• A Novel Versatility of Catalase I as a Dioxygenase for Indole - ring - opening Dioxygenation
• The Artificial Evolution of an Enzyme by Random Mutagenesis : The Development of Formaldehyde Dehydrogenase
• Characterizations of topological dimension by use of normal sequences of finite open covers and Pontrjagin-Schnirelmann theorem
• Photoinduced Reduction of Methylviologen with TiO_2/Polymer Films
• Characterizations of topological dimension by use of normal sequences of finite open covers and Pontrjagin-Schnirelmann theorem
• A CHARACTERIZATION OF ω_1-STRONGLY COUNTABLE-DIMENSIONAL METRIZABLE SPACES
• Substituent Effects on the Photosolvolyses of 2-Chloropropiophenones in 2,2,2-Trifluoroethaol
• One Dimensional Modeling of Radio Frequency Electric Field Penetration into Magnetized Plasmas
• Scaling Laws of Lissajous Acceleration for Electrodeless Helicon Plasma Thruster

スポンサーリンク