High-level Production of Hyperthermophilic Cellulase in the Bacillus brevis Expression and Secretion System
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概要
- 論文の詳細を見る
A hyperthermophilic cellulase derived from Pyrococcus horikoshii was successfully produced with the Bacillus brevis host-vector system. The production of the recombinant enzyme was increased about 20-fold (to a level of 100 mg per liter) by the insertion of certain amino acid such as alanine and peptides like AEEAADP between the carboxyl end of signal peptide and the N-terminus of the mature cellulase. These recombinant cellulases had the same characteristics as that of the cellulase expressed in Escherichia coli.
- 社団法人 日本農芸化学会の論文
- 2004-01-23
著者
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Kashima Yasuhiro
Special Division For Human Life Technology National Institute Of Advanced Industrial Science And Tec
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Udaka Shigezo
Nagoya University
関連論文
- Alkyl Hydroperoxide Reductase Dependent on Thioredoxin-Like Protein from Pyrococcus horikoshii
- High-level Production of Hyperthermophilic Cellulase in the Bacillus brevis Expression and Secretion System