LC-ESI/MSによる法化学的薬物・毒物分析
スポンサーリンク
概要
- 論文の詳細を見る
This paper presents a liquid chromatography-electrospray ionization-mass spectrometric (LC-MS) screening and identification procedures for drugs and toxic substances from forensic biological materials such as postmortem blood, urine, and tissues (so-called general unknown screening or systematic toxicological analysis). Gas chromatoraphy-mass spectrometry (GC-MS) has been the method of choice for toxicological analysis of volatile substances while non-volatile and/or thermally labile substances need LC-MS for identification. To develop the LC-MS procedures for forensic analysis, mass spectra library and retention indices, like in the case of GC-GM, are required. Retention indices were determined according to the retention times on the chromatographic separation using a Waters Symmetry C18 (particle size 3.5 μm), 2.1 mm × 150 mm column with a gradient of methanol and 10 mM ammonium acetate (pH 6.8) delivered at a 0.2 mL/min flow rate. Retention times of substances were then converted to retention indices based on those of uracil, phenol, methylparaben (methyl-p-hydroxybenzoate), ethylparaben (ethyl-p-hydroxybenzoate), propylparaben (propyl-p-hydroxybenzoate), butylparaben (butyl-p-hydroxybenzoate), and heptylparaben (heptyl-p-hydroxybenzoate) in the retention index standard mixture. Mass spectra library was made applying two different cone voltages (20 V and 60 V) both in the positive and negative ion modes for in-source fragmentation due to collision-induced dissociation. Mass spectra obtained presented the molecular-related ion and number of specific fragment ions sufficient for identification. The combination of the retention index, molecular-related ion and fragment ions has made LC-MS a preferred method for general unknown screening in the field of forensic toxicology to GC-MS.
- 2004-02-01