Modification of Cysteine Residue in Peptides by a Fluorescent Reagent for Complete Sequence Analyses Using Post-Source Decay Method in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (プロテオミクス特集号)
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概要
- 論文の詳細を見る
In the sequence analyses of antigen peptides from nucleoprotein in influenza virus, it was very difficult to obtain the sufficient numbers of product ions using the post-source decay (PSD) method in MALDI-TOF-MS. Fluorescent modification of the thiol group of the cysteine residue in the target peptides using 5-iodoacetamide fluorescein was introduced for the PSD measurement. The fluorescently-labeled peptides gave sufficient product ions for complete sequence analyses in the PSD spectrum, which leads to the complete sequence analyses of the peptides with cysteine residue.
- 日本質量分析学会の論文
- 2003-10-01
著者
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Nakagawa Masatoshi
Biological Information Research Center National Institute Of Advanced Industrial Science And Technol
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YAMAGAKI Tohru
Department of Biochemistry, Saitama University
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NAKANISHI Hiroshi
Biological Information Research Center, National Institute of Advanced Industrial Science and Techno
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Nakanishi Hiroshi
Biological Information Research Center National Institute Of Advanced Industrial Science And Technology
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