Vitamin B_6 Antagonists Alter the Function and Ultrastructure of Mice Endothelial Cells

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概要

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• Vitamin B6 is necessary for normal membrane function and stability. Here we studied both the function and ultrastructure of aortic and arterial endothelial cells (ECs) in vitamin B6 deficient mice induced by vitamin B6 antagonists, 4-deoxypyridoxine⋅HCl (dPN⋅HCl), and isonicotinylhydrazide (INH). Mice were fed with normal laboratory chow and divided into three groups according to their drinking water. Mice in group I had distilled water and served as a control; group II had 0.1mg dPN HCl/mL H2O; and group III had 0.4mg INH/mL H2O. After 5 mo, plasma concentrations of B6 vitamers pyridoxal-5'-phos-phate (PLP) and pyridoxal (PL) were analyzed by HPLC. With the arachidonic acid (AA) as a precursor, prostacyclin (PGI2) production from ECs assayed by thin-layer chromatography (TLC) was used as an indicator of endothelial function. Aorta and arteriole from foot pad were removed, stained with osmium tetraoxide, and examined under transmission electron microscopy (TEM) to study the ultrastructure of ECs. The results showed that plasma con-centrations of PLP, PL, and total B6 were the lowest for mice fed with INH, followed by that with dPN⋅HCl, compared with that of control. PGI2 production was paralleled with the plasma vitamin B6 status, with the lowest level for INH, followed by the dPN-treated group. Abnormalities in the ultrastructure of ECs were found in both dPN⋅HCl and INH groups, in-cluding cells detached from underlying elastic tissue, with prominent pinocytotic vesicles and swelling and or indistinct cristae of mitochondria. These results suggest that vitamin B6 antagonists induce a deficient status that alters the function and the ultrastructure of ECs detrimental to vascular disease.

• 財団法人 学会誌刊行センターの論文
• 2000-08-01

著者

• Chang S‐j

  National Cheng Kung Univ. Tainan Twn

• Chang Sue-joan

  Department Of Biology National Cheng Kung University

関連論文

• Vitamin B_6 Down-Regulates the Expression of Human GPIIb Gene
• The effects of pilocarpine-induced status epilepticus on oxidative stress/damage in developing animals
• Vitamin B_6 Status of Breast-Fed Infants in Relation to Pyridoxine・HCl Supplementation of Mothers
• Vitamin B_6 Antagonists Alter the Function and Ultrastructure of Mice Endothelial Cells

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