Detection by PCR of the Tyzzer's Disease Origanism (Clostiridium piliforme) in Feces

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概要

• 論文の詳細を見る

• We examined whether the Tyzzer’s disease organism, Clostridium piliforme, could be detected in feces by PCR. If the organism could be detected in feces, a diagnosis could be made without sacrifice of the animal. Using the RT strain of C. piliforme, we found that a C. piliforme band could be detected when there were ≥ 1 × 10° bacteria present in the PCR solution, but the presence of fecal extract in the solution depressed the sensitivity 10 fold. Nevertheless, we could detect the C. piliforme-specific band in fecal extracts from rats in a naturally infected colony, and concluded that the use of PCR to detect C. piliforme DNA in fecal extracts would be a useful diagnostic technique.

• 社団法人　日本実験動物学会の論文
• 2002-10-01

著者

• Okada Eiko

  Taiho Pharmaceutical Co. Ltd. Drug Safety Research Lab.

• Furukawa Toshinori

  Research Facilities For Laboratory Animal Science Hiroshima University School Of Medicine

• FURUMOTO Kayo

  SHIMIZU Laboratory Supplies Co., Ltd.

• FUJIEDA Mitsuhiro

  Taiho Pharmaceutical Co., Ltd. Drug Safety Research Lab.

• Fujieda Mitsuhiro

  Taiho Pharmaceutical Co. Ltd. Drug Safety Research Lab.

• Furumoto Kayo

  Shimizu Laboratory Supplies Co. Ltd.

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• Detection by PCR of the Tyzzer's Disease Origanism (Clostiridium piliforme) in Feces

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