Scarce adenylation in bacteriophage T4 mRNAs

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概要

• 論文の詳細を見る

• The degradation of mRNA is crucial for the rapid shift of bacteriophage T4 gene expression from early to late. The present study was conducted to investigate whether T4 mRNA is polyadenylated or not, because polyadenylation is known to facilitate the degradation of mRNA in Escherichia coli cells. Total RNA extracted from T4-infected cells was subjected to self-circularization or intermolecular ligation by T4 RNA ligase, and a region containing the 3-5 junction was amplified by RT-PCR. Cloning and sequencing as well as the length distribution of amplified DNA fragments revealed no adenines at the 3-ends of uvsY and soc RNAs. The present result suggests that T4 mRNA is not significantly adenylated.

• 日本遺伝学会の論文
• 2002-08-25

著者

• YONESAKI Tetsuro

  Department of Biological Sciences, Graduate School of Science, Osaka University

• Yonesaki Tetsuro

  Department Of Biological Sciences Graduate School Of Science Osaka University

• Yonesaki T

  Department Of Biology Graduate School Of Science Osaka University

関連論文

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• Scarce adenylation in bacteriophage T4 mRNAs
• Role of Escherichia coli Hfq in late-gene silencing of bacteriophage T4 dmd mutant
• Opposite roles of the dmd gene in the control of RNase E and RNase LS activities
• Carboxyl Terminal Segment of T4 Gene 32 Protein is Dispensable in Viv^o
• AbpA and AbpB provide anti-phage activity in Escherichia coli

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