Phaseolus calcaratus の異なる2品種における in vitro での迅速なマイクロプロパゲーション

概要

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A successful protocol for rapid proliferation of adventitious shoot from hypocotyl and leaf explants of two cultivars of Phaseolus calcaratus cv., RBL-1 and RBH-35 is described. A high frequency of multiple shoot buds was obtained directly from cut ends of the explants on MS medium supplemented with different (1-10 mgl-1) concentrations of auxins viz. 2, 4-dichlorophenoxyacetic acid (2, 4-D), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) alone and in combination with cytokinin, 6-benzyladenine (BA). Lower concentrations of auxins (0.1 and 0.5mgl-1) alone and in combination with different concentrations of BA were in general found to be the best for multiple shoot differentiation within 3-4 weeks of culture. Rooting of in vitro regenerated shoots was induced by NAA (0.1mgl-1) alone or a combination of IBA and BA (0.1+0.1mgl-1). Regeneration was much higher from hypocotyl than from leaf.
日本植物細胞分子生物学会の論文
1995-04-01