CD44 and the ERM Family in Bone Cells

スポンサーリンク

概要

• 論文の詳細を見る

• We describe here the immunohistochemical localization of CD44 (hyaluronate receptor) and the ezrin-radixin-moesin (ERM) family (actin binding proteins) in bone cells and discuss possible mechanisms for the organization of their cytoskeletons.<BR>In the cells of osteoblast-lineage, active osteoblasts showed immunoreactivity to CD44 only on their cytoplasmic processes in the bone matrix. They showed degrees of immunoreactivity to radixin and moesin on the cytoplasmic side of their basolateral plasma membrane, but not in their cytoplasmic processes. On the other hand, osteocytes demonstrated intense immunolabeling to CD44, showing scarcely any immunoreactivity to the ERM family. In osteoclasts, intense immunoreactivity to CD44 was detected on their basolateral plasma membranes. The plasma membranes of the clear zone and the ruffled border were not immuno-labeled with CD44. As for the ERM family, the basolateral plasma membrane of the osteoclasts was stained with anti-moesin monoclonal antibody, but not with anti-ezrin or anti-radixin antibody. Thirty minutes after the administration of calcitonin, osteoclasts did not show either clear zones or ruffled borders. CD44 and moesin were colocalized all along their plasma membranes, including the region facing the bone surface.<BR>These findings suggest that: 1) the CD44moesin-actin filament system is involved in the cytoskeletal organization of osteoclasts and their cell-polarity; and 2) other mechanisms, rather than the CD44 and the ERM family, may be involved in the cells of osteoblast-lineage.

• 日本組織細胞化学会の論文
• 1997-04-01

著者

• OZAWA Hidehiro

  First Department of Oral Anatomy, Niigata University School of Dentistry

• Ozawa Hidehiro

  First Department Of Oral Anatomy Niigata Unibersity Faculty Of Dentistry

• NAKAMURA Hiroaki

  First Department of Oral Anatomy, Niigata University School of Dentistry

• Nakamura Hiroaki

  岡山大学 歯 第1口腔解剖

• Nakamura Hiroaki

  First Department Of Oral Anatomy Niigata University School Of Dentistry

関連論文

• Distribution of actin filaments, non-muscle myosin, M-Ras, and extracellular signal-regulated kinase (ERK) in osteoclasts after calcitonin administration
• Ultrastructural and immunohistochemical studies of medullary bone calcification, with special reference to sulphated glycosaminoglycans
• Experimental Glaucoma Model in the Rat Induced by Laser Trabecular Photocoagulation After an Intracameral Injection of India Ink
• Histochemical study of alveolar bone remodeling in op/op mice by the administration of macrophage-colony stimulating factor
• Immunohistochemical localization of parathyroid hormone/parathyroid hormone-related peptide receptor in rat tibiae
• Organic components of crystal sheaths in bones
• Effect of intermittent administration of human parathyroid hormone (1-34) on the mandibular condyle of ovariectomized rats
• Changes in type I, II, and X collagen immunoreactivity of the mandibular condylar cartilage in a naturelly aging rat model
• Age-related histological changes in rat mandibular condyle
• Ultrastructure and Immunolocalization of Transforming Growth Factor-Beta in Chondrification of Murine Ligamentous Fibroblasts and Endochondral Calcification Induced by Recombinant Human Bone Morphogenetic Protein-2
• W1-3 Application of EELS : mechanisms of biological calcification and localizational regulation of calcium and phosphorus
• The localization of CD44 and moesin in osteoclasts after calcitonin administration in mouse tibiae
• CD44 and the ERM Family in Bone Cells

もっと見る 閉じる

スポンサーリンク