Effect of Fixation with Reduced Osmium Tetroxide upon the Antigenicity of Liver Catalase and Erythrocyte Esterase D

概要

論文の詳細を見る
Effect of reduced OsO4 fixation on the antigenicity of rat liver catalase and erythrocyte esterase D was investigated. After fixation with mixture of 4% paraformaldehyde and 0.2% glutaraldehyde, rat liver and erythrocytes were post-fixed with reduced osmium (0.1% to 1.0%) for 1 hr. Some erythrocytes were fixed with 0.5% reduced osmium for various times (10 min to 60min). The antigenicity of both enzymes was expressed as labeling density (gold particles/μm2 of peroxisomes or erythrocytes). Fixation with reduced OsO4 greatly affected the antigenicity of both enzymes. Approximately 80% of catalase antigenicity was lost at 0.4% reduced OsO4, whereas about 80% of esterase D antigenicity was lost at 0.2%. Positive contrast of membrane structures clearly appeared at 0.6% OsO4. About 80% loss of the esterase D antigenicity was observed at 10min after fixation with 0.5% reduced OsO4. Afterwards, the antigenicity decreased very slowly. The data indicates that at the concentration of reduced OsO4 which stains the membrane structures positive contrast, 80% of the antigenicity is lost in the cases of catalase and esterase D.
日本組織細胞化学会の論文
1996-08-01