Cytochrome Oxidase Activity of Individual Mitochondrion as Quantified by Platinum-Diaminobenzidine Reaction with Energy Dispersive X-ray Analyzer

概要

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The platinum diaminobenzidine (Pt-DAB) reaction was characterized by the oxidative polymerization of DAB with a concurrent incorporation of Pt atoms into the polymerization product in the presence of cytochrome oxidase activity. It conformed to the Michaelis-Mentens formula showing its enzymatic nature. It was specific to the oxidase as proved by the suppressive effects due to some inhibitors and heat. It permitted the quantification of the oxidase activity of an individual mitochondrion and its constituent part by energy dispersive X-ray analysis (EDAX) owing to the incorporated Ptatoms. To elucidate the gradient in the oxidase activity along the radial axis of a liver lobule, the local activity difference between the hepatocytes in the peripheral region (PE) adjacent to the triad and those in the central region (CN) close to the central vein was quantified at individual mitochondrial level. The mitochondria in the PE hepatocytes were found 1.5 times stronger in activity <I>per</I> area of the reactive site than those in the CN hepatocytes.
日本組織細胞化学会の論文
1995-06-01