Antioxidative Activity of Natural Isorhapontigenin

概要

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Isorhapontigenin (ISOR), isolated from Belamcanda chinensis, is a derivative of stilbene. Its chemical structure is very similar to that of resveratrol, with a potent antioxidative effect. In the present study, we investigated the antioxidative activity of ISOR in vitro. Oxidative damage of rat liver microsomes, brain mitochondria and synaptosomes was induced by Fe2+-Cys, VitC-ADP-Fe2+ and H2O2, respectively. The formation of malondialdehyde (MDA), decrease of reduced glutathione (GSH) and increase of ultra-weak chemiluminescence during the lipid peroxidation process were determined. In addition, the characteristic ultra-weak chemiluminescence of oxidative DNA damage induced by CuSO4-Phen-VitC-H2O2 system was studied. The results showed that ISOR significantly inhibited MDA formation in liver microsomes, brain mitochondria and synaptosomes induced by Fe2+-Cys. Also, ISOR markedly prevented the decrease of GSH in mitochondria and synaptosomes induced by H2O2 and the increase of ultra-weak chemiluminescence during lipid peroxidation induced by VitC-ADP-Fe2+ as well as oxidative DNA damage induced by CuSO4-Phen-VitC-H2O2. The effects of ISOR at 10–5 and 10–6 mol/L on the MDA formation and decrease of GSH were similar to that of the classical antioxidant vitamin E (10–4 mol/L). It may be concluded that ISOR possessed potent antioxidative activity and was much more potent than vitamin E.
社団法人日本薬理学会の論文
2001-09-01