Production of Germfree Mice by Embryo Transfer

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概要

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• We applied the embryo transfer technique to germfree (GF) mouse production. Embryos harvested from superovulated mice were transferred aseptically, in a sterile environment, to the uterus of GF recipient females which had been mated with vasectomized GF males. One of the recipients became pregnant and delivered offspring. Sterility tests confirmed that the vasectomized males, newborns, recipient female mice, embryo-containing culture media, and the inside of the vinyl film isolator were germfree. These results suggest that the embryo transfer technique can be successfully applied to the production of GF mice.

• 社団法人　日本実験動物学会の論文

著者

• OKAMOTO Masanori

  Laboratory Animal, Radiation Safety Center, Natl. Inst. Radiol. Sci.

• Matsumoto Tsuneya

  Laboratory Animal And Plant Sciences National Institute Of Radiological Sciences

• Okamoto Masanori

  Laboratory Animal And Plant Sciences National Institute Of Radiological Sciences

関連論文

• Analysis of Radiation-induced Germline Mutation Using SNP Marker
• Production of Germfree Mice by Embryo Transfer
• Decrease and Recovery of Ejaculated Sperm Concentration after Acute Testicular Gamma-Ray Irradiation in Cynomolgus Monkeys (Macaca fascicularis).

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