Partial Hepatectomy of Marmoset: Clinical and Pathological Effects and Utility in Microsomal Enzyme Analysis
スポンサーリンク
概要
- 論文の詳細を見る
Liver biopsy based on a partial hepatectomy technique (shearing) was performed in 10 common marmosets (Callithrix jacchus). This is a preliminary study to evaluate the effects of drugs on hepatic microsomal enzymes: cytochrome P-450 and T4 uridine diphosphate glucuronyl transferase (T4-UDPGT), by comparing post-treatment values with pre-treatment values individually with a limited number of animals. The effects of the biopsy on clinical findings and liver pathology were evaluated during the first 5 post-surgical weeks. Although the plasma aspartate aminotransferase (AST) activities tended to decrease from 1 to 4 weeks post-surgery, no abnormality was noted in clinical sign, body weight, the hematocrit value or other blood chemical values. At necropsy, adhesion of the sheared site of the liver to the parietal peritoneum or the small intestine was evident in 2 of the 4 marmosets. Microscopic examination revealed focal fibrosis in the liver, but it was localized around the sheared site. Based on the above results, it was concluded that liver biopsy must be performed more than one month before administration of the drug to be tested. The biopsy samples and the whole liver samples obtained at autopsy were subjected to analysis of microsomal protein content, cytochrome P-450 content and T4-UDPGT activity. In comparison with the values from the whole liver samples, those from the biopsy samples showed no significant difference. Furthermore, there was a significant correlation rather than difference between matched values. This suggested that partial hepatectomy is a useful method for obtaining pretreatment values in liver biochemistry to evaluate the effects of drug-treatment in individual animals.
- 社団法人 日本実験動物学会の論文
著者
-
TOYOTA Naoto
Mitsubishi Chemical Safety Institute Ltd.
-
Tanaka K
Mitsubishi Chemical Corporation Yokohama Research Center
-
KURATA Yoshimasa
Mitsubishi Chemical Safety Institute Ltd.
-
MAKINODAN Futoshi
Mitsubishi Chemical Safety Institute Ltd.
-
MATSUMOTO Junko
Mitsubishi Chemical Safety Institute Ltd.
-
TANAKA Keisuke
Mitsubishi Chemical Corporation, Yokohama Research Center
-
Kurata Yoshimasa
Mitsubishi Chemical Medience Corp.
-
Makinodan Futoshi
Mitsubishi Chemical Medience Corporation
関連論文
- 22D-10-4 Induction of replicative DNA synthesis (RDS) in rat livers following sibgle inhalation exposure to carbon tetrachloride.
- Evaluation of turbidimetric immunoassay for urine albumin of laboratory animals (TEST METHODS) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- P7-29 Clinical pathological characteristics of a spontaneous dwarf mutation in BriHan : WIST@Jcl(GALAS) rat(IMMUNE AND ALLERGIC SYSTEM/KIDNEYS AND URINARY EXCRETION SYSTEM/CYTOTOXICITY/OTHERS-1)(GENERAL SESSION BY POSTER PRESENTATION)(Proceedings of the 3
- Morphological and Endocrinological Characteristics of the Endocrine Systems in Wistar Hannover GALAS Rats Showing Spontaneous Dwarfs
- P-50 Effect of Aniline on Motor Activity in Rats.(Proceedings of the 27th Annual Meeting)
- Partial Hepatectomy of Marmoset: Clinical and Pathological Effects and Utility in Microsomal Enzyme Analysis
- P-52 The Examination of Quantitative Analysis using Image Analyzer on the Expression of Glial Fibrillary Acidic Protein (GFAP) in the Brain of Rats Received Trimethyltin.(Proceedings of the 27th Annual Meeting)
- P10-16 Increased osteoid volume and iron deposition of the Iumbar vertebra in a chronic cadmium toxicosis model of cynomolgus monkeys.
- 21D-04-1 Quantitative Analysis of Bone Lesion in Ovariectomized Cynomolgus Monkeys Given Repeated Intravenous Injections of CdCl_2.
- OCCURRENCE OF TOXICITY AND CELL PROLIFERATION AFTER A SINGLE GAVAGE ADMINISTRATION OF CHLOROFORM TO MALE F344 RATS
- 21D-01-2 Colloidal Silver Staining on Cellulose Acetate Membrane Electrophoresis for Analysis of Urinary Proteins in Rats and Dogs.
- ACUTE TOXICITY OF CADMIUM IN RATS WITH OR WITHOUT CADMIUM PRETREATMENT
- 302 EFFECTS OF CADMIUM CHLORIDB ON THE BLOOD AND URINARY EXAMINATIONS IN OVARIECTOMIZED RATS
- Evaluation of measurement and differentiation of nucleated cell in bone marrow using hematology analyzer XT-2000iV for laboratory animals(Test methods, Proceedings of the 32nd Annual Meeting)
- P8-35 Basic evaluation of hematology analyzer XT-2000i for laboratory animals(STATISTICAL AND TEST METHODS/LOCAL STIMURATION)(GENERAL SESSION BY POSTER PRESENTATION)(Proceedings of the 31st Annual Meeting)
- Metabolism of di (2-ethylhexyl) phthalate (DEHP) : comparative study in juvenile and fetal marmosets and rats
- Metabolism of di (2-ethylhexyl) phthalate (DEHP) : comparative study in juvenile and fetal marmosets and rats
- Metabolite profiling and identification in human urine after single oral administration of DEHP