レトロトランスポゾン・プロ-ブを用いた栽培イネのDNAフィンガ-プリンティング〔英文〕

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概要

• 論文の詳細を見る

• Cloned sequences of three retrotransposons of rice, Tos1-1, Tos2-1 and Tos3-1, were used as molecular genetic markers to distinguish the cultivars of Asian and African rice, Oryza sativa and Oryza glaberrima. DNAs of six cultivars each of Indica and Japonica types of O. sativa were analyzed after digestion with four different restriction enzymes. Indica cultivars could be distinguished from each other by any of three types of one probe-one restriction enzyme combination. Although the hybridization patterns were similar among Japonica cultivars, these cultivars could be distinguished from each other by one type of one probe-one enzyme combination. Five cultivars of O. glaberrima examined were also distinguished from each other by using one probe-one enzyme combination. The results shown here indicate that retrotransposon-mediated fingerprinting is an efficient method to distinguish or identify the cultivars of rice. Retrotransposonmediated fingerprinting should become a general method, because retrotransposons are ubiquitous in plant species and retrotransposon probes can easily be obtained from any plant species.

• 日本遺伝学会の論文
• 1993-06-25

著者

• Hirochika Hirohiko

  Department Of Molecular Biology National Institute Of Agrobiological Resources

• Kikuchi Fumio

  Institute Of Space And Aeronautical Science University Of Tokyo

• FUKUCHI Atushi

  Agricultural Research Laboratories, Takeda Chemical Industries, Ltd.

• Kikuchi Fumio

  Institute Of Plant Breeding University Of Tsukuba

• Fukuchi Atushi

  Agricultural Research Laboratories Takeda Chemical Industries Ltd.

• 福地 淳

  Agricultural Research Laboratories, Takeda Chemical Industries, Ltd.

• 菊池 文雄

  Institute of Plant Breeding, University of Tsukuba

• 広近 洋彦

  Department of Molecular Biology, National Institute of Agrobiological Resources

関連論文

• Two Novel Nuclear Genes, OsSIG5 and OsSIG6, Encoding Potential Plastid Sigma Factors of RNA Polymerase in Rice : Tissue-Specific and Light-Responsive Gene Expression
• The slender Rice Mutant, with Constitutively Activated Gibberellin Signal Transduction, Has Enhanced Capacity for Abscisic Acid Level
• Characterization of a Rice Chlorophyll-Deficient Mutant Using the T-DNA Gene-Trap System
• 植物ゲノムに遍在するTy1-copiaグル-プレトロトランスポゾン〔英文〕
• Identification of a Putative Voltage-Gated Ca^-permeable Channel (OsTPC1) Involved in Ca^ Influx and Regulation of Growth and Development in Rice
• A Mutation Study of the DNA Binding Domain of Human Papillomavirus Type11 E2 Protein^1
• レトロトランスポゾン・プロ-ブを用いた栽培イネのDNAフィンガ-プリンティング〔英文〕
• ACTIVATION OF RETROTRANSPOSONS BY STRESS
• Convergence of the ACM Finite Element Scheme for Plate Bending Problems

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