A linkage map of the turnip sawfly Athalia rosae (Hymenoptera:Symphyta) based on random amplified polymorphic DNAs

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概要

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• A linkage map was constructed for the sawfly, Athalia rosae (Hymenoptera), based on the segregation of random amplified polymorphic DNA (RAPD) markers and a visible mutation, yellow fat body (yfb). Forty haploid male progeny (20 yfb and 20 +) from a single diploid female parent (yfb/+) were examined. Sixty-one of the 180 arbitrary primers tested by polymerase chain reaction (PCR) produced one or more RAPD bands. A total of 79 RAPD markers were detected. Of these, seven showed significant deviation from the expected 1:1 ratio, and were therefore excluded from further analysis. The remaining 72 RAPD markers and the marker mutation, yfb, were subjected to linkage analysis. Sixty RAPD markers and the yfb marker were organized into 16 linkage groups, spanning a distance of 517.2 cM. Twelve RAPD markers showed no linkage relationship to any group. Thirteen gel-purified RAPD bands were cloned and sequenced to generate the sequence-tagged sites (STSs). A single locus was represented by two markers, with one of them having a short internal deletion.

• 日本遺伝学会の論文

著者

• Lee J

  Developmental Mechanisms Laboratory Developmental Biology Department National Institute Of Agrobiolo

• Lee Jae

  Graduate Institute of Ferrous Technology, Pohang University of Science and Technology

• Lee J

  Department Of Biology College Of Natural Sciences Daegu University

• Lee Jae

  Developmental Biology Department National Institute Of Agrobiological Sciences

• Sumitani Megumi

  Department of Biological Sciences, Tokyo Metropolitan University

• Hatakeyama Masatsugu

  Developemental Mechanisms Laboratory Developmental Biology Department National Institute Of Agrobiol

• Hatakeyama Masatsugu

  Department Of Biology Faculty Of Science Kobe University

• Lee Jae

  Graduate School Of Science And Technology Kobe University

• NISHIMORI Yoshikatsu

  Graduate School of Science and Technology, Kobe University

• SUMITANI Megumi

  Graduate School of Science and Technology, Kobe University

• OISHI Kugao

  Graduate School of Science and Technology, Kobe University

• Lee Jae

  Graduate Institute Of Ferrous Technology Pohang University Of Science And Technology

• Hatakeyama M

  Developmental Mechanisms Laboratory Developmental Biology Department National Institute Of Agrobiolo

• Oishi Kugao

  Graduate School Of Science And Technology Kobe University

• Sumitani Megumi

  Department Of Biological Sciences Tokyo Metropolitan University

• Nishimori Yoshikatsu

  Graduate School Of Science And Technology Kobe University

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