Novel point mutations in mitochondrial 16S rRNA gene of Chinese hamster cells

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概要

• 論文の詳細を見る

• To know the nature and mechanisms of spontaneous mutations in mitochondrial DNA (mtDNA), we determined, by direct cycle sequencing, the nucleotide sequence of the 3 terminal region of the mitochondrial 16S rRNA gene from chloramphenicol-resistant (CAP-R) mutants isolated in Chinese hamster V79 cells. Four different base substitutions were identified in common for the six CAP-R mutants. All mutations were heteroplasmic. One A to G transition was mapped at a site within the putative peptidyl transferase domain, the target region for chloramphenicol, and one G to A transition and two T to G transversions were located within the two different segments which form the stems of the hairpin loop structures attached to this key domain in the predicted secondary structure of 16S rRNA. The mutations detected in this study do not map to the same sites where CAP-R mutations were found previously in mammalian cells. Allele specific-PCR analyses revealed that all four mutations occurred on a single mutant-DNA molecule, but not on several ones independently. Together with the other previous reports, our data suggest that spontaneous mtDNA mutations may not be caused exclusively by oxidative DNA damage at least in 16S rRNA gene.

• 日本遺伝学会の論文

著者

• Hashiguchi Kazunari

  Laboratory Of Molecular Genetics Biology Division Kyoto University Of Education

• Ikushima Takaji

  Laboratory Of Molecular Genetics Biology Division Kyoto University Of Education

• IKUSHIMA TAKAJI

  Laboratory of Genetics, Faculty of Agriculture, Kyoto University

関連論文

• Nucleotide changes in mitochondrial 16S rRNA gene from different mammalian cell lines
• Novel point mutations in mitochondrial 16S rRNA gene of Chinese hamster cells
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