Cloning and characterization of a mariner-like element in the silkworm, Bombyx mori

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概要

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• We have identified a mariner-like element (MLE) in the genome of the silkworm, Bombyx mori. The cloned MLE, desiqnated as BmMLE, contained an ORF that was interrupted by insertions, stop codons, and frameshifts. The complete ORF sequence was estimated by sequencing the PCR products. The hypothetical protein coded by this ORF showed 34% homology to the Mos1 element of Drosophila mauritiana, and is phylogenetically very close to MLE of Hyalophora cecropia. BmMLE is present in 80 to 100 copies in the B. mori genome. Many BmMLEs with different lengths were identified by Southern blot analysis and LA PCR of the genomic DNA. The distribution of these BmMLEs differed in geographic races.

• 日本遺伝学会の論文

著者

• TAMURA Toshiki

  Department of Allergology, The Institute of Medical Science, the University of Tokyo

• Tomita Shuichiro

  Department Of Insect Genetics And Breeding National Institute Of Sericultural And Entomological Scie

• Tomita Shuichiro

  Department Of Insect Genetics And Breeding National Institute Of Sericultural And Entomological Scie

• Sohn Bong

  National Sericulture And Entomology Research Institute

• Tamura Toshiki

  Department Of Insect Genetics And Breeding National Institute Of Sericultural And Entomological Scie

• Tamura Toshiki

  Department Of Allergology The Institute Of Medical Science The University Of Tokyo

関連論文

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• Suppressed induction of mycobacterial antigen-specific T_h1-type CD4^+ T cells in the lung after pulmonary mycobacterial infection
• Yeast FLP recombinase-mediated excision in cultured cells and embryos of the silkworm, Bombyx mori (Lepidoptera : Bombycidae)
• Cloning and characterization of a mariner-like element in the silkworm, Bombyx mori

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