Identification of the sex of Oriental white stork, Ciconia boyciana, by the polymerase chain reaction based on its sex chromosome-specific DNA sequences

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概要

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• Southern blotting of HindIII-digested genomic DNAs from the female and male Oriental white stork (Ciconia boyciana) with the EE0.6 probe cloned from the W chromosome of chicken (Gallus domesticus) produced a 3.0-kb W chromosome-derived band specific to the female and a 3.5-kb Z chromosome-derived band common to both sexes. These two genomic fragments were cloned and the counter-part sequences to that of EE0.6 in these fragments, XH0.6 and XH0.6RSM (XH0.6-related sequence in the male), respectively, were subcloned. Nucleotide sequences of XH0.6 and XH0.6RSM showed 92% identity. PCR using a set of primer sequences from XH0.6, which differed several nucleotides from those in XH0.6RSM, amplified an about 300-bp genomic sequence only from the female C. boyciana. This method was applied successfully to identify the sex of individual young birds of C. boyciana, an endangered special natural monument in Japan and whose sexes are unidentifiable from their external morphology.

著者

• Mizuno Shigeki

  Laboratory Of Molecular Biology Department Of Applied Biological Chemistry Faculty Of Agriculture To

• MURATA Koichi

  Kobe Oji Zoo

• HOSODA Takahisa

  Tama Zoological Park

• ITOH Yuichiro

  Laboratory of Molecular Biology, Department of Applied Biological Chemistry, Faculty of Agriculture,

• OGAWA Akira

  Laboratory of Molecular Biology, Department of Applied Biological Chemistry, Faculty of Agriculture,

• Itoh Yuichiro

  Laboratory Of Molecular Biology Department Of Applied Biological Chemistry Faculty Of Agriculture To

• Hosoda T

  Tama Zoological Park Tokyo Jpn

• Ogawa Akira

  Laboratory Of Molecular Biology Department Of Applied Biological Chemistry Faculty Of Agriculture To

• Murata Koichi

  Kobe Municipal Oji Zoo

• MIZUNO Shigeki

  Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University

関連論文

• The Primary Structure of Cassowary (Casuarius casuarius) Goose Type Lysozyme(Biochemistry & Molecular Biology)
• Identification of the sex of Oriental white stork, Ciconia boyciana, by the polymerase chain reaction based on its sex chromosome-specific DNA sequences
• Molecular cell biological studies on the structure, function and evolution of avian sex chromosomes(Abstracts of the 48th Annual Meeting of the Society of Chromosome Research)
• Purification and Characterization of Goose Type Lysozyme from Cassowary (Casuarlus casuarius) Egg White
• Determination of the complete nucleotide sequence and haplotypes in the D-loop region of the mitochondrial genome in the Oriental white stork, Ciconia boyciana
• Multiple Actin-Related Proteins of Saccharomyces cerevisiae Are Present in the Nucleus^1
• Actin-Related Proteins in the Nucleus and Their Involvement in the Function of Chromatin
• Two Isoforms of a Human Actin-Related Protein Show Nuclear Localization and Mutually Selective Expression between Brain and Other Tissues
• 家蚕におけるNd-s変異の遺伝分析〔英文〕
• First Report to Thelazia sp. from a Captive Oriental White Stork (Ciconia boyciana) in Japan.
• Multiple Actin-Related Proteins of Saccharomyces cerevisiae Are Present in the Nucleus.

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