Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase
スポンサーリンク
概要
- 論文の詳細を見る
Glycolate(GL)-based polyesters were for the first time produced in the recombinant Escherichia coli fatty acid β-oxidation pathway reinforcing mutant LS5218, using extracellularly added GL as a monomer precursor. Cells expressing a Ser325Thr/Gln481Lys mutant of polyhydroxyalkanoate synthase (PhaC1STQK) from Pseudomonas sp. 61-3, propionyl-CoA transferase from Megasphaera elsdenii and enoyl-CoA hydratase from Pseudomonas aeruginosa grown on GL and dodecanoate were found to produce novel copolymers of GL with 3-hydroxyalkanoates (3HAs) (C4-C12), P(GL-co-3HA), with a weight-average molecular weight of 34000. The 1H NMR analyses of the copolymer revealed the incorporation of GL units into the polymer chain. This result demonstrates that PhaC1STQK polymerized glycolyl-CoA as a monomer substrate. Additionally, the novel lactate(LA)-based polyester P(LA-co-3HA) was produced by substituting GL with LA, indicating that the method is versatile and allows the production of a variety of biopolymers.
- 2011-12-10
論文 | ランダム
- 庄内弁に関するデジタルコンテンツ制作について--卒業研究課題を通して
- JPEGデータ構造の概要
- Scilab/Scicosによる制御系設計とシミュレーション
- A new design of multi-limb robot with regular octahedron trunk and verification of its movement
- 純ニッケル材の表面に及ぼすNa2SO4電解酸化水の影響