Dynamic Behavior and Spontaneous Differentiation of Mouse Embryoid Bodies on Hydrogel Substrates of Different Surface Charge and Chemical Structures
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概要
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Differentiation of embryoid bodies (EBs) into particular cell lineages has been extensively studied. There is anincreasing interest in the effect of soft hydrogel scaffolds on the behavior of EBs, such as the initial adhesion,dynamic morphology change, and differentiation. In this study, without adding any other bioactive factors inthe serum-containing medium, dynamic behaviors of mouse EBs loaded on the surface of hydrogels withdifferent surface charge and chemical structures are investigated. EBs adhered quickly to negatively chargedpoly(sodium p-styrene sulfonate) (PNaSS) hydrogels, which facilitates EBs spreading, migration, and differentiationinto three germ layers with high efficiency of cardiomyocytes differentiation, similar to that on gelatincoated polystyrene (PS) culture plate. While on neutral poly(acrylamide) (PAAm) hydrogels, EBs maintained theinitial spherical morphology with high expression of pluripotency-related markers in the short culture periods,and then showed the significantly greater levels of selected endoderm markers after long-time culture. EBscultured on negatively charged poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS) gelsdemonstrated the analogous behaviors with that of neutral PAAm gels at early differentiation phase (day 4 + 1).Then, their adhesion, spreading and differentiation were quite similar to that on negatively charged PNaSS gels.The correlation between surface properties of hydrogels and EBs differentiation was discussed.
- 2011-08-25
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