Determination of Bisphosphonates by Ion-pair HPLC

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The optimization of the two bisphosphonates (disodium pamidronate and zoledronic acid) separation by ion?suppression and ion?pair high?performance liquid chromatography is described. The influence of the eluent pH, composition of the mobile phase and the concentration of complexing ion?pairing agents was investigated. This developed method provides high separation and specificity to bisphosphonate analysis. In quantitative analysis in the optimized conditions (10 mM n?amylamine or n?butylamine; 14% acetonitrile in 0.1 M sodium phosphate buffer, pH=7.0), the method showed good linearity (r=0.9999?0.9998) and sufficient sensitivity (10^?7 ?10^?8g/ml). It can be easily and conveniently adopted for the routine quality control analysis.