Fluorescence ratiometric detection of ligand-induced receptor internalization using extracellular coiled-coil tag-probe labeling.
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概要
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We report a new method for the detection of ligand-induced receptor internalization by fluorescence ratiometric imaging of pH in endosomes in combination with a recently developed posttranslational labeling system based on the formation of a heterodimeric coiled-coil structure. The N-terminus of the β2-adrenergic receptor expressed on the cell surface was doubly labeled with pH-sensitive fluorescein and pH-insensitive tetramethylrhodamine. A significant increase in the tetramethylrhodamine-to-fluorescein fluorescence intensity ratio was observed after incubation with agonists in a concentration-dependent manner. This simple and accurate method of detecting the agonistic activity of receptors will be useful for high-throughput screening of drug candidates.
- 2011-07-21
論文 | ランダム
- 熊本1946年と86年(町の対照) (時代の対照--1946年と1986年)
- わたしのつくりたい教育番組 (テレビがつくる日本)
- 怪奇絵のなかの青春・竹中英太郎推論 (続・思想史に学ぶ(主題))
- タンクローの確執--死にざまと生きざま (児童文学の再検討(特集))
- 70年わたしの姿勢 ('60〜'70・ふたたび市民としての抵抗(特集))