Endosomal escape and the knockdown efficiency of liposomal-siRNA by the fusogenic peptide shGALA
スポンサーリンク
概要
- 論文の詳細を見る
An siRNA that specifically silences the expression of mRNA is a potential therapeutic agent for dealing with many diseases including cancer. However, the poor cellular uptake and bioavailability of siRNA remains a major obstacle to clinical development. For efficient delivery to tumor tissue, the pharmacokinetics and intracellular trafficking of siRNA must be rigorously controlled. To address this issue, we developed a liposomal siRNA carrier, a multi-functional nano device (MEND). We describe herein an approach for systemic siRNA delivery to tumors by combining the MEND system with shGALA, a fusogenic peptide. In cultured cell experiments, shGALA-modification enhanced the endosomal escape of siRNA encapsulated in a polyethylene glycol modified MEND (PEG-MEND), resulting in an 82% knockdown of the target gene. In vivo systemic administration clarified that the shGALA-modified MEND (shGALA-MEND) showed 58% gene silencing in tumor tissues at a dose of 4 mg of siRNA/kg body weight. In addition, a significant inhibition of tumor growth was observed only for the shGALA-MEND and no somatic or hepatic toxicity was observed. Given the above data, this peptide-modified delivery system, a shGALA-MEND has great potential for the systemic delivery of therapeutic siRNA aimed at cancer therapy.
論文 | ランダム
- 荻野喜弘著『筑豊炭鉱労資関係史』
- 家族看護過程--家族という土壌を耕すケア理論と実際(21)援助方法(その10)家族成員間の関係性に働きかける援助(4)
- 家族看護過程--家族という土壌を耕すケア理論と実際(第12回)援助方法(その1)個々の家族成員に対する援助
- 家族看護過程--家族という土壌を耕すケア理論と実際(10)家族のアセスメント(その3)情報をどのように引き出し判断するか
- 家族看護過程(9)家族のアセスメント(その2)看護上の問題の明確化