Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus.
スポンサーリンク
概要
- 論文の詳細を見る
Cellobiose 2-epimerase (EC 5.1.3.11) was first identified in 1967 as an extracellular enzyme that catalyzes the reversible epimerization between cellobiose and 4-O-β-d-glucopyranosyl-d-mannose in a culture broth of Ruminococcus albus 7 (ATCC 27210T). Here, for the first time, we describe the purification of cellobiose 2-epimerase from R. albus NE1. The enzyme was found to 2-epimerize the reducing terminal glucose moieties of cellotriose and cellotetraose in addition to cellobiose. The gene encoding cellobiose 2-epimerase comprises 1170 bp (389 amino acids) and is present as a single copy in the genome. The deduced amino acid sequence of the mature enzyme contains the possible catalytic residues Arg52, His243, Glu246, and His374. Sequence analysis shows the gene shares a very low level of homology with N-acetyl-d-glucosamine 2-epimerases (EC 5.1.3.8), but no significant homology to any other epimerases reported to date.
- Elsevier Inc.の論文
- 2007-08-31
Elsevier Inc. | 論文
- Effect of lycopene and beta-carotene on peroxynitrite-mediated cellular modifications.
- Expression of hormone genes and osmoregulation in homing chum salmon: A minireview.
- Anti-parallel membrane topology of two components of EbrAB, a multidrug transporter.
- Incidence and Risk of Postherpetic Neuralgia after Varicella Zoster Virus Infection in Hematopoietic Cell Transplantation Recipients: Hokkaido Hematology Study Group
- Insertion (21;8)(q22;q22q22) : a masked t(8;21) in a patient with acute myelocytic leukemia