2011年度神奈川大学総合理学研究所共同研究助成論文線虫Caenorhabditis elegansをもちいた繊毛の本数が減少する変異体のスクリーニング

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短報　2011 年度神奈川大学総合理学研究所共同研究助成論文Cilia and flagella are microtubule‐based protruding organelles observed on cell surfaces．In mammals，dysfunction of cilia leads to various types of disease（ciliopathies），which are often caused by loss of intraflagellar transport（IFT）．While the molecular basis of IFT has been well studied，little is known about the mechanisms underlying the early phase of ciliogenesis．The number of cilia，which varies according to the cell characteristics，is determined in the early phase．In Caenorhabditis elegans， sensory neurons are associated with one or two cilia, and the number is not stochastically but strictly determined according to the cell fate. For example，one of the head sensory neuron classes，ADL，is double ciliated，whereas another sensory neuron class，ASE，is single ciliated．To dissect the molecular mechanisms that determine the number of cilia，we screened for mutants with an abnormal cilia number．ADL cilia were observed by using a strain in which ADL is labeled by green fluorescent protein．By screening，we obtained three mutants in which ADL is single ciliated．
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