2010年度神奈川大学総合理学研究所共同研究助成論文成長に伴うオニテナガエビ造雄腺の組織像の変化

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2010年度神奈川大学総合理学研究所共同研究助成論文Male sexual characteristics in crustaceans are induced by androgenic gland hormone (AGH), which is produced in a male-specific organ, the androgenic gland (AG). In this study, we conducted histochemical studies on the AGs in immature and mature males of the giant freshwater prawn Macrobrachium rosenbergii. In order to confirm the position of the AG, gene expression of an insulin-like AG factor (IAG), which is expressed specifically in the AG, was examined by in situ hybridization. Specific hybridization signals were detected only in the AG cells attached to the external wall of the terminal ampoule. This localization of IAG mRNA is the same as that of the AG in M. rosenbergii reported previously. By histological staining, apparent AG cells were observed in immature M. rosenbergii as well as mature prawns. This result indicates that AGH regulates not only male morphotypic differentiation at the immature stage, but spermatogenesis at the mature stage.