電気刺激法によるアオサとアマノリのプロトプラストの属内融合〔英文〕

概要

論文の詳細を見る
Isolated algal protoplasts of 1. Ulva pertusa (sterile mutant) and U. conglobata, 2. Porphyra yezoensis normal and P. yezoensis green type, were electrically fused. The protoplasts from each alga were mixed together with its algal partner in a 1:1 ratio in low conductivity electrofusion solution at a density of 1×10⁵⁻⁶ cells/ml. Protoplasts were aligned into short chains in high frequency (1 MHz) alternate current (AC) field and sudsequently fused by the application of a single short duration direct current (DC) pulse. Protoplasts aligned at 200 V for 10 s and 40 V for 20 s yielded maximum pairs about 25 and 40% in Ulva and Porphyra respectively. The application of 20-25 μ s duration DC pulse of 200 V resulted optimum binary fusion percentages about 12% in Ulva and whereas 250 V of 40 μ s duration yielded maximum fusions about 16% in Porphyra. The application of a high intensity DC pulse (> 300 V of 30 μ s duration for Ulva and > 350 V of 40 μ s duration for Porphyra) to the aligned protoplasts induced protoplast lysis.
長崎大学水産学部の論文