pH Measurement Using Dual-Wavelength Fluorescent Ratio by Two-Photon Excitation for Mitochondrial Activity
スポンサーリンク
概要
- 論文の詳細を見る
A mitochondrion has a pH gradient between the two sides of its inner membrane in order to produce adenosine triphosphate (ATP). Because ATP depletion causes numerous diseases, the measurement of the pH value around the mitochondrion is expected to clarify the mechanism of these diseases. In this study, a dual-wavelength pH-sensitive dye was excited by two-photon absorption initiated using a femtosecond pulse laser. In addition, fluorescence from the dye was directly collected from the fluorescent point using the collection-mode probe of a scanning near-field optical microscope. By this proposed method, a pH calibration curve was obtained from the fluorescent intensity ratio of the dye solution, and temporal pH variations with 0.1 s time resolution following the addition of acid were observed. Moreover, mitochondrial activity on the basis of the pH changes was successfully observed in three different mitochondrial densities.
- 2012-11-25
著者
-
Umeda Norihiro
Department Of Industrial Mechanical Engineering Faculty Of Technology Tokyo University Of Agricultur
-
Ohta Yoshihiro
Department Of Anesthesiology And Intensive Care Hokkaido University School Of Medicine
-
Ohta Yoshihiro
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
-
Onojima Takumi
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
-
Umeda Norihiro
Department of Mechanical System Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
-
Iwami Kentaro
Department of Mechanical System Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
-
Kanazashi Yasuaki
Department of Mechanical System Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
-
Li Yongbo
Department of Mechanical System Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
関連論文
- Dew Condensation Sensor Using Quartz Tuning Fork Coated with Polystyrene Sphere Self-Assembled Monolayer
- "Syn-Effect"in the Isomerization of (E)-α-Fluorovinylic Sulfones to the Corresponding Allylic Sulfones under Basic Conditions
- Sputter Deposited Zinc Oxide Photoconductive Antenna on Silicon Substrate for Sub-Terahertz Time-Domain Spectroscopy
- Automated Anesthesia Record Keeping by Engineering Work Station Four Years Experience
- Localized Thermal Processing with a Laser-trapped and Heated Metal Nanoparticle
- Abnormal Cerebral Blood Flow Associated with Antiphospholipid Antibody Syndrome : Four Case Reports
- Absolute Positioning of Scanning Probe Microscope Tip by Two-Wavelength Synthetic Method
- Beam Shaping Optics for YAG Laser Processing Fabricated by Computerized Numerical Control Lathe
- Liquid Pressure Varifocus Lens
- Cardiopulmonary Bypass Produces Non-uniform Pressure Differences between Femoral and Radial Arteries
- Optical Isolator for Orthogonally Polarized Two-Frequency Laser
- Heterogeneous Populations of Adrenal Fasciculata Cells Examined with Ca^ Signaling and Immunostaining : Single Cell Imaging
- Multi-Wavelength Mueller Matrix Polarimeter
- Surface Profiling Using the Photothermal Displacement Method
- Surface Segregation of Well-defied N-Substituted Hyperbranched Polyamides in Linear Polymer Matrix
- Effects of Progesterone on Intracellular Ca^ Levels of Immortalized Hypothalamic Neurons (GT1-7) : Fluorescence Imaging Study
- Effect of Acidosis on Vasoconstrictors and Vasodilators in Rat Aortic Rings
- 1P399 Suppression of ROS production by UCP2 in neuronal cell subjected to oxidative stress(15. Cellular signal transduction,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- pH Measurement Using Dual-Wavelength Fluorescent Ratio by Two-Photon Excitation for Mitochondrial Activity
- Birefringence Polarimeter Using Dual LiNbO3 Electrooptic Crystal Modulators
- Publisher's Note: ``Birefringence Polarimeter Using Dual LiNbO3 Electrooptic Crystal Modulators''
- Manipulation of Metal Nanoparticles using Fiber-Optic Laser Tweezers with a Microspherical Focusing Lens
- Optical Dew Sensor Using Surface Plasmon Resonance of Periodic Ag Nanostructure
- Direct Alignment of Liquid Crystal Molecules Using an Atomic Force Microscope