Signal-to-Background Ratio Improvement for Single-Molecule Spectroscopy Using Tube Lens to Reduce the Effective Detection Region
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概要
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We demonstrate the improvement in the signal-to-background ratio of squaraine-derived rotaxane dye molecules embedded in poly(methyl methacrylate) polymer film by reducing the area of the effective detection region in a confocal optical microscope system. It is found that a long-focal-length tube lens combined with the use of a pinhole can evidently minimize the effective detection volume as well as the background photons, and that the signal-to-background ratio of single molecules is improved to 24 experimentally.
- 2012-08-15
著者
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Jia Suotang
State Key Laboratory Of Quantum Optics And Quantum Optics Devices College Of Physics And Electronics
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Xiao Liantuan
State Key Laboratory Of Quantum Optics And Quantum Optics Devices College Of Physics And Electronics
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WANG Xiaobo
State Key Lab of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Scienc
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Zhang Guofeng
State Key Laboratory of Quantum Optics and Quantum Optics Devices, College of Physics and Electronics Engineering, Shanxi University, Taiyuan 030006, China
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Chen Ruiyun
State Key Laboratory of Quantum Optics and Quantum Optics Devices, College of Physics and Electronics Engineering, Shanxi University, Taiyuan 030006, China
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Jia Suotang
State Key Laboratory of Quantum Optics and Quantum Optics Devices, College of Physics and Electronics Engineering, Shanxi University, Taiyuan 030006, China
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Xiao Liantuan
State Key Laboratory of Quantum Optics and Quantum Optics Devices, College of Physics and Electronics Engineering, Shanxi University, Taiyuan 030006, China
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Wang Xiaobo
State Key Laboratory of Quantum Optics and Quantum Optics Devices, College of Physics and Electronics Engineering, Shanxi University, Taiyuan 030006, China
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