Measurement of Macrophage Adhesion at Various pH Values by Optical Tweezers with Backward-Scattered Detection

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概要

• 論文の詳細を見る

• Optical tweezers have emerged as a powerful tool with broad applications in biology and physics. In force-measuring applications, the trapped bead position is usually accurately determined by forward-scattered detection. The current study discusses both backward-scattered detection and forward-scattered detection related to the linear detection range for a 3 μm bead and the distance between the two laser system focuses, confirming the optimum positions of the two focuses. The result indicates that the linear detection range of backward-scattered detection is longer than the forward-scattered one. Finally, this work investigates real-time adhesion force measurements between human macrophages and 3 μm trapped beads coated with lipopolysaccharides at various pH values by optical tweezers with backward-scattered detection.

• Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
• 2010-07-25

著者

• Hsu Long

  Institute and Department of Electrophysics, National Chiao-Tung University, 1001 Ta Hsueh Road, Hsinchu, Taiwan 30010, R.O.C.

• Su Yi-Jr

  Institute and Department of Electrophysics, National Chiao-Tung University, 1001 Ta Hsueh Road, Hsinchu, Taiwan 30010, R.O.C.

関連論文

• Recovery Based Nanowire Field-Effect Transistor Detection of Pathogenic Avian Influenza DNA (Special Issue : Solid State Devices and Materials (1))
• Study of the Line Optical Tweezers Characteristics Using a Novel Method and Establishing a Model for Cell Sorting
• Measurement of Macrophage Adhesion at Various pH Values by Optical Tweezers with Backward-Scattered Detection

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