Ultrasensitive Detection of Cymbidium Mosaic Potexvirus Using a Single-Wall Carbon Nanotube-Functionalized Quartz Crystal Microbalance
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概要
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We have developed an ultrasensitive, convenient, real-time platform for detecting Cymbidium mosaic potexvirus (CymMV) based on single-wall carbon nanotube (SWNT)-functionalized quartz crystal microbalance (QCM) sensors. Functionalization was achieved by coating the QCM electrode with SWNTs, followed by 1,1$'$-carbonyldiimidazole-activated Tween 20 (CDI–Tween 20) modification and conjugation of antibodies. Sensitivity was enhanced from 2.18 to 11.5 Hz ng-1 when 0.1 μg mL-1 CymMV was applied. The low limit of detection of SWNT-functionalized QCM sensors was improved from 2.08 to 0.502 ng. The SWNT-functionalized QCM sensor was successfully used to quantify the amount of CymMV contained in infected orchid leaves. Compared to enzyme-linked immunosorbent assay (ELISA), SWNT-functionalized QCM sensors are fast, economical, and ultra-sensitive, with comparable sensitivities. The current study demonstrates the application of QCM sensors as a convenient platform to detect and quantify CymMV.
- 2010-10-25
著者
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Chiou Jin-chern
Institutet Of Electrical And Control Engineering National Chiao Tung Univ.
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Hung Yao-ching
Department Of Obs Gyn China Medical University Hospital
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Huang Guewha
Institute Of Nanotechnology National Chiao Yung University
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Wang Hui-Liang
Department of Biotechnology, National Kaohsiung Normal University, 62 Shen Jhong Road, Kaohsiung 824, Taiwan, Republic of China
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Chen Yu-Shiun
Institute of Nanotechnology, Department of Materials Science and Engineering, National Chiao Tung University, 1001 University Road, EE137, Hsinchu 300, Taiwan, Republic of China
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Huang Hung-Shu
Institute of Nanotechnology, Department of Materials Science and Engineering, National Chiao Tung University, 1001 University Road, EE137, Hsinchu 300, Taiwan, Republic of China
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Huang Li-Chia
Departments of Obstetrics and Gynecology, School of Medicine, China Medical University and Hospital, 91 Hsueh-Shih Road, Taichung 404, Taiwan, Republic of China
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Chiou Jin-Chern
Institute of Electrical Control Engineering, National Chiao Tung University, 1001 University Road, EE772, Hsinchu 300, Taiwan, Republic of China
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