Kinetics of Microtubule–AtMAP65-1 Bond Studied with Dual-Optical Tweezers
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概要
- 論文の詳細を見る
The unbinding force of microtubule–AtMAP65-1 bond is measured with dual-optical tweezers. Force histograms reveal quantized force distributions under physiological condition and with 100 mM NaCl treatment. Based on Bell-Evans-model of multiple bonds, histograms are fitted and kinetic parameters are obtained. Under the force loading rate of 9 pN/s, the most probable unbinding forces for the single bond are $16.8\pm 0.8$ and $14.7\pm 0.8$ pN under the physiological and treatment conditions, respectively. Position of transition state of the bond is given to be $\Delta x=2.1\pm 0.1$ nm both with and without treatment of NaCl. Intrinsic dissociation rate constants $k_{0}$ are $0.0010\pm 0.0002$ and $0.0020\pm 0.0005$ s-1 corresponding to without and with NaCl treatment, which means that 100 mM NaCl shortens the natural lifetime of the bond by 50%. In addition, the activation barrier also changes by 3.4% after treatment with NaCl, which indicates that NaCl makes the dissociation of the bond easier.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2007-11-15
著者
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Cheng Bingying
Optical Physics Laboratory Institute Of Physics And Center For Condensed Matter Physics Chinese Acad
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Yuan Ming
State Key Laboratory Of Plant Physiology And Biochemistry ; Department Of Plant Sciences College Of
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Yuan Ming
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China
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Qu E
Optical Physics Laboratory, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, P. O. Box 603, Beijing 100080, China
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Guo Honglian
Optical Physics Laboratory, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, P. O. Box 603, Beijing 100080, China
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Xu Chunhua
Optical Physics Laboratory, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, P. O. Box 603, Beijing 100080, China
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Li Zhaolin
Optical Physics Laboratory, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, P. O. Box 603, Beijing 100080, China
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Zhang Daozhong
Optical Physics Laboratory, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, P. O. Box 603, Beijing 100080, China
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Cheng Bingying
Optical Physics Laboratory, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, P. O. Box 603, Beijing 100080, China
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