Application of Scanning Probe Microscopy to Genetic Analysis
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概要
- 論文の詳細を見る
We are developing an integrated technique involving of nanometer-size dissection of chromosome fragments by atomic force microscopy (AFM) and direct detection of the location of genome library clones by scanning near-field optical/atomic force microscopy (SNOM/AFM). The locations of nucleus organizer regions (NORs) on barley chromosomes and a bacterial artificial chromosome (BAC) clone were successfully detected by SNOM/AFM. Nanometer-scale dissection of silkworm pachytene chromosomes was also performed by AFM, and we succeeded in three successive dissection events of the chromosome region approximately 250 nm apart from each other. If this type of integrated method can be established in the near future, we will easily obtain the nucleotide sequences with positional information on chromosomes, which lead to a time- and cost-saving genome analysis technique.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2006-03-15
著者
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Suetsugu Yoshitaka
National Institute of Agrobiological Sciences
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Narukawa Junko
National Institute of Agrobiological Sciences
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Sasou Megumi
National Food Research Institute Naro
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Ohtani Toshio
National Food Research Institute
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Kuwazaki Seigo
National Food Research Institute
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Yamamoto Kimiko
National Insitute Of Agrobiological Sciences
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Tsukamoto Kazumi
National Food Research Institute Naro
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YOSHINO Tomoyuki
National Food Research Institute
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Takahashi Hirokazu
National Agricultural Research Center For Hokkaido Region (narch)
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Sugiyama Shigeru
National Food Res. Inst.
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Yoshino Tomoyuki
National Food Research Institute, 2-12-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
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Takahashi Hirokazu
National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan
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Sugiyama Shigeru
National Food Research Institute, 2-12-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
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Ohtani Toshio
National Food Research Institute, 2-12-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
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