Effects of Extremely Low Frequency Magnetic Field on Neurite Outgrowth of PC12 and PC12D Cells and Evaluation by Image Analysis

概要

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A pheochromocytoma cell (PC12), and its derivative (PC12D), differentiate to nervelike cells in culture with the nerve growth factor (NGF) and forskolin respectively. We introduced a morphological factor $\sigma=L/2(\pi A)^{1/2}$ for quantitating neurite outgrowth under a microscope in the presence of extremely low-frequency (ELF) magnetic fields for 22 hours, where $L$ and $A$ are the contour length and the area of the cells in clump determined using an image-analysis system. ELF magnetic fields $B_{1}$ were generated with a single coil or double coils in Helmholtz configuration together with static fields $B_{0}$ of $-53$, $-20$ and 67 μT. $\sigma$ increased with increasing NGF or forskolin level at $B_{0}=-53$ μT (geomagnetism), in agreement with the cytometric observation of micrographs. With the addition of an AC field $B_{1}$ at 60 Hz (100 μT $>$ $B_{1}$ $>$ 3 μT rms) to $B_{0}$, neurite outgrowth represented by $\sigma$ was depressed for PC12 and stimulated for PC12D. We discuss the cyclotron resonance and the ion parametric resonance models.
2004-05-15