Increased Phosphorylation of Ca²⁺ Handling Proteins as a Proarrhythmic Mechanism in Myocarditis
スポンサーリンク
概要
- 論文の詳細を見る
Background:Because fatal arrhythmia is an important cause of death in patients with myocarditis, we investigated the proarrhythmic mechanisms of experimental autoimmune myocarditis.Methods and Results:Myocarditis was induced by injection of 2 mg porcine cardiac myosin into the footpads of adult Lewis rats on days 1 and 8 (Myo, n=15) and the results compared with Control rats (Control, n=15). In an additional 15 rats, 6 mg/kg prednisolone was injected into the gluteus muscle before the injection of porcine cardiac myosin on days 1 and 8 (MyoS, n=15). Hearts with myocarditis had longer action potential duration (APD), slower conduction velocity (CV; P<0.01 vs. Control), higher CV heterogeneity, greater fibrosis, higher levels of immunoblotting of high-mobility group protein B1, interleukin 6 and tumor necrosis factor-α proteins. Steroid treatment partially reversed the translations for myocarditis, CV heterogeneity, reduced APD at 90% recovery to baseline, increased CV (P<0.01), and reversed fibrosis (P<0.05). Programmed stimulation triggered sustained ventricular tachycardia in Myo rats (n=4/5), but not in controls (n=0/5) or Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor (KN93) treated Myo rats (n=0/5, P=0.01). CaMKII autophosphorylation at Thr287 (201%), and RyR2 phosphorylation at Ser2808 (protein kinase A/CaMKII site, 126%) and Ser2814 (CaMKII site, 21%) were increased in rats with myocarditis and reversed by steroid.Conclusions:The myocarditis group had an increased incidence of arrhythmia caused by increased phosphorylation of Ca2+handling proteins. These changes were partially reversed by an antiinflammatory treatment and CaMKII inhibition. (Circ J 2014; 78: 2292–2301)
- 一般社団法人 日本循環器学会の論文
著者
-
Park Sungha
Cardiology Division Department Of Internal Medicine Yonsei University College Of Medicine
-
Joung Boyoung
Cardiology Division Department Of Internal Medicine Yonsei University Medical College
-
PAK Hui-Nam
Cardiology Division, Department of Internal Medicine, Yonsei University College of Medicine
-
LEE Moon-Hyoung
Cardiology Division, Department of Internal Medicine, Yonsei University Medical College
-
Park Hyelim
Cardiology Division, Internal Medicine, Yonsei University
-
Hwang Hye
Cardiology Division, Yonsei Cardiovascular Center and Cardiovascular Research Institute, Yonsei University College of Medicine
-
Lim Jisoo
Cardiology Division, Yonsei University College of Medicine
-
Oh Sujung
Cardiology Division, Yonsei University College of Medicine
-
Park Hyewon
Cardiology Division, Yonsei University College of Medicine
-
Lee Dajeong
Cardiology Division, Yonsei University College of Medicine
関連論文
- Delayed Enhancement Magnetic Resonance Imaging Is a Significant Prognostic Factor in Patients With Non-Ischemic Cardiomyopathy
- Effect of Vessel Size on Lipid Content of Coronary Plaques Assessed by Integrated Backscatter Intravascular Ultrasound
- Rescuing an Entrapped Guidewire Using a Tornus^[○!R] Catheter
- Soluble Fms-Like Tyrosine Kinase-1 and the Progression of Carotid Intima-Media Thickness : 24-Month Follow-up Study
- Magnetic Dispersion of the Late Repolarization in Brugada Syndrome
- Gender-Related Difference in Left Ventricular Diastolic Elastance During Exercise in Patients With Diabetes Mellitus
- Time Course of Recovery of Left Ventricular Filling Pressure After Exercise in Healthy Subjects
- PE-450 Left Ventricular Concentric Remodeling is Associated with Early LV Systolic and Diastolic Dysfunction in Patients with Treated Essential Hypertension(Hypertension, clinical-05, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-305 Echocardiographic Epicardial Adipose Tissue is Related to High Sensitivity C-reactive Protein in Hypertensive Patients(Atherosclerosis, clinical-02, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-054 Genetic Polymorphisms in the Pregnancy-Associated Plasma Protein-A are Associated with Acute Myocardial Infarction(Acute myocardial infarction, basic-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Insulin Resistance Is Associated with Arterial Stiffness in Nondiabetic Hypertensives Independent of Metabolic Status
- Pediatric Radiofrequency Catheter Ablation : Sedation Methods and Success, Complication and Recurrence Rates
- Efficacy of Clotinab in Acute Myocardial Infarction Trial-ST Elevation Myocardial Infarction (ECLAT-STEMI)
- Prognostic Value of Change in Red Cell Distribution Width 1 Month After Discharge in Acute Decompensated Heart Failure Patients
- Endotracheal Exposure of Particulate Matter Induces Arrhythmia via Oxidative Stress and Calcium Calmodulin Kinase II Activation
- Long-Term Outcome of Intensive Education as Treatment of Vasovagal Syncope: Median 3 Year Follow-Up
- Early Reperfusion in STEMI Influences Transmural Injury Characteristics and Electrocardiographic Manifestations
- Heart Rate Acceleration of Subsidiary Pacemaker by β-Adrenergic Stimulation
- Fate of Preoperative Atrial Fibrillation After Correction of Atrial Septal Defect
- Heat Shock Protein, αB Crystallin, Can Prevent the Arrhythmogenic Effect of Ambient Particulate Matter by Attenuating the Repolarization Gradient and Triggered Activity
- Protective Effects of Ranolazine on Air Pollutant-Induced Ventricular Arrhythmia through Late INa Inhibition
- Effect of a Single Bolus Injection of Low-Dose Hydrocortisone for Prevention of Atrial Fibrillation Recurrence After Radiofrequency Catheter Ablation
- Fate of Preoperative Atrial Fibrillation After Correction of Atrial Septal Defect
- Effect of a Single Bolus Injection of Low-Dose Hydrocortisone for Prevention of Atrial Fibrillation Recurrence After Radiofrequency Catheter Ablation
- Increased Phosphorylation of Ca²⁺ Handling Proteins as a Proarrhythmic Mechanism in Myocarditis