Observation of Glycolytic Metabolites in Tumor Cell Lysate by Using Hyperpolarization of Deuterated Glucose

スポンサーリンク

概要

• 論文の詳細を見る

• Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of 13C6-glucose-d7 and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin–lattice relaxation time T1. 13C6-Glucose-d7 exhibited a T1 that was over ten times as long as that of 13C6-glucose, and metabolic NMR studies of hyperpolarized 13C6-glucose-d7 using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.

• 公益社団法人 日本薬学会の論文

著者

• Akakabe Mai

  Science Research Center, Kochi University

• Tsuda Masashi

  Center for Advanced Marine Core Research, Kochi University

• Fukushi Eri

  Research Faculty of Agriculture, Hokkaido University

• Kumagai Keiko

  Science Research Center, Kochi University

• Tsuda Masayuki

  Science Research Center, Kochi University

• Kawabata Jun

  Research Faculty of Agriculture, Hokkaido University

• Abe Takamasa

  Oxford Instruments KK

• Ichikawa Kazuhiro

  Innovation Center for Medical Redox Navigation, Kyushu University

関連論文

• Observation of Glycolytic Metabolites in Tumor Cell Lysate by Using Hyperpolarization of Deuterated Glucose

スポンサーリンク